工厂化生产香肠肠衣腐烂黑斑中嗜盐细菌的分离鉴定及其特征特性  

Isolation,Identification and Characteristics of Halophilic Bacteria in Decayed Black Spot of Sausage Casing

作　　者：牟丽丽[1] 涂云华 Syren de Hoog 刘涛华[1] 吕倩[1] 王颜颜[1] 陈燕[1] 康颖倩[1] 

机构地区：[1]贵州医科大学微生物学教研室,贵州贵阳550004 [2]CBS荷兰皇家科学院真菌生物多样性中心,荷兰乌得勒支85167

出　　处：《贵州农业科学》2016年第1期93-97,共5页Guizhou Agricultural Sciences

基　　金：国家自然科学基金地方项目"贵州省病原需氧放线菌的系统分类研究及其属间快速鉴别诊断试剂盒的研发"(31260029);贵州省国际科技合作计划项目"白酒丢糟及锅底水水解循环利用技术""[黔科合外G字(2014)7006];贵州省社会发展科技攻关项目"贵州省放线菌资源的开发及临床放线菌属间快速鉴别诊断试剂盒的研发"[黔科合SY字(2011)3017];贵阳市科技局科技创新平台计划项目"姜黄纳米保健产品科技创新平台的建设"[筑科合同2012303]

摘　　要：为工厂化生产的相关食品及农产品储存过程中微生物的鉴定及抑菌对策提供理论依据,采用高盐察氏液体培养基（Czapek Dox Broth）梯度盐浓度培养法,CDA（Czapek Dox Agar）及TSA（Trypticase Soy Agar）固体培养基进行分离纯化,16SrRNA基因测序,邻接法构建16SrRNA序列的系统发育树等方法,对工厂生产香肠肠衣腐烂黑斑中嗜盐细菌进行分离鉴定,研究其特征特性。结果表明：25%~30%盐浓度固体培养基中分离到3株嗜盐细菌（Gyfl1,Gyfl2,Gyfl3）,经分子学分析鉴定Gyfl1为Halomonas variabilis,Gyfl2为Chromohalobacter israelensis,Gyfl3为Chromohalobacter canadensis;经药敏试验,庆大霉素、卡那霉素、利福平、红霉素和新霉素等常见抗生素均能抑制嗜盐菌株Gyfl1、Gyfl2和Gyfl3,而利福平抑菌效果更明显。The halophilic bacteria in decayed black spot of sausage casing were isolated and identified by the gradient salt concentration method（Czapek Dox Broth liquid medium）,CDA（Czapek Dox Agar）and TSA（Trypticase Soy Agar）solid media,and the characteristics of the identified halophilic bacteria was analyzed by using 16 SrRNA gene sequencing and building phylogenetic tree of 16 SrRNAsequence to provide the theoretical basis for microbial identification and bacteriostat measures during the storage process of agricultural products and related foods produced by industrialization.Results：Three halophilic bacteria strains（Gyfl1,Gyfl2 and Gyfl3）are successfully isolated on the solid medium with 25% ~30%salt concentration. Gyfl1,Gyfl2 and Gyfl3 strains are identified as Halomonas variabilis,Chromohalobacter israelensis and Chromohalobacter Canadensis by molecular analysis respectively.The drug sensitive test shows that common antibiotics of gentamicin,kanamycin,rifampicin,erythromycin,and neomycin all can inhibit Gyfl1,Gyfl2 and Gyfl3strains but the antibacterial effect of rifampicin is the highest among common antibiotics.

关 键 词：嗜盐细菌 香肠肠衣 系统发育树 抗生素敏感 

分 类 号：TS205.3[轻工技术与工程—食品科学]