葫芦素B诱导G_2/M期阻滞抑制人前列腺癌DU145细胞增殖并诱导细胞凋亡研究  

Cucurbitacin B induces G_2/M arrest and apoptosis in human prostate cancer DU145 cells

作　　者：张萌[1] 边志刚[2] 张毅[1] 王佳贺[1] 阚亮[1] 何平[1] 

机构地区：[1]中国医科大学附属盛京医院干诊科,辽宁沈阳110004 [2]中国医科大学附属盛京医院鼻科,辽宁沈阳110004

出　　处：《中国医刊》2016年第2期63-67,共5页Chinese Journal of Medicine

基　　金：辽宁省科学技术计划项目(2013225021)

摘　　要：目的探讨葫芦素B对人前列腺癌DU145细胞增殖及凋亡的影响。方法体外培养DU145细胞,MTT法检测不同浓度葫芦素B对DU145细胞的增殖抑制作用;流式细胞仪检测细胞周期;Hoechst 33258核染色观察细胞核的形态变化;采用流式细胞术检测细胞凋亡率;Western blot检测葫芦素B对DU145细胞周期调节蛋白Cyclin B1表达的影响。结果葫芦素B对DU145细胞的增殖有抑制作用,且呈剂量、时间依赖性;葫芦素B使DU145细胞发生G_2/M期阻滞;可见细胞核染色质浓缩、核固缩、核碎裂等典型的凋亡改变;流式细胞仪检测结果显示,葫芦素B诱导DU145细胞凋亡,呈剂量依赖性;葫芦素B使DU145细胞Cyclin B1蛋白表达降低。结论葫芦素B通过诱导G_2/M期阻滞抑制人前列腺癌DU145细胞增殖并诱导细胞凋亡。Objective To study the influence of Cucurbitacin B on the proliferation and apoptosis in human prostate cancer DU145 cells. Method Human prostate cancer DU145 cells were cultured in vitro. The growth inhibitory effect of Cucurbitacin B on DU145 cells was detected by MTT assay. The cel1 cycle distribution was examined by flow cytometry. The changes of the nucleus were investigated by Hoechst 33258 staining. Apoptosis rate was determined by flow cytometry analysis. The expression of cell cycle related protein cyclin B1 was detected by Western blot assay. Result The growth of DU145 cells was inhibited by Cucurbitacin B in a dose-and time-dependent manner. Cucurbitacin B induced G2/M arrest in DU145 cells. Typical morphological hallmarks of apoptosis, including condensation of chromatin, karyopyknosis and nuclear fragmentation were observed. Flow cytometry analysis showed that Cucurbitacin B could induce DU145 cells apoptosis in a dose-dependent manner. Western blot assay showed that cyclin B1 protein was down-regulated after Cucurbitacin B treatment. Conclusion Cucurbitacin B could inhibit the proliferation of DU145 cells through induction of G2/M arrest and cell apoptosis.

关 键 词：葫芦素B 前列腺癌 G_2/M期阻滞 凋亡 

分 类 号：R737.25[医药卫生—肿瘤]