星点设计-效应面法优化白芍提取工艺  

Optimization of Extraction Process for Paeoniae radix Alba by Central Composite DesignResponse Surface Methodology

作　　者：魏词 万蒞 王红[2] 黄国军[1] 

机构地区：[1]湖北省妇幼保健院药剂科,武汉430070 [2]湖北中医药大学药学院,武汉430065

出　　处：《医药导报》2016年第2期174-176,共3页Herald of Medicine

基　　金：湖北省教育厅科研项目(D20101806)

摘　　要：目的优化白芍的提取工艺。方法以芍药苷、白芍总苷及干膏得率为指标,采用归一法处理,运用星点设计-效应面法对白芍提取工艺进行优化。结果优选的最佳提取工艺为加9倍量85.00%乙醇提取2次,每次100min。3批验证性实验表明实际值与预测值基本一致。结论应用星点设计-效应面法优化白芍提取工艺,实验值与预测值吻合度高,所建立的模型预测性良好,优选的提取工艺合理、稳定、可行。Objective To optimize the extraction process of Paeoniae radix Alba. Methods Extraction rates of paeoniflorin and total glucosides from Paeoniae radix Alba and yield of dry extract were served as research indexes. Extraction process was optimized by central composite design-response surface methodology. Results Optimum condition of extraction technology was to extract 2 times with 9-fold of 85. 00% ethanol for 100 min each time. Results of the three batches of confirmatory test were accordant with the predicted values. Conclusion The observed and predicted values were close to each other,and the optimum formulation by CCD-RSM was successful. The established model has a reliable predictability. The optimum extraction process is reasonable,stable and feasible.

关 键 词：白芍 芍药苷 芍药总苷 星点设计-效应面法 提取工艺 

分 类 号：R289.5[医药卫生—方剂学] TQ284.2[医药卫生—中药学]