人工栽培蛹虫草致病木霉的鉴定及生物学特性分析  

Identification and Biological Analysis on Pathogenic Trichoderma from Cultivated Cordyceps militaris

作　　者：李娟[1,2] 刘晓红[1,2] 王旭[1] 季长波[1] 孙书伟[1] 宋洁[1,2] 

机构地区：[1]辽东学院农学院,辽宁丹东118003 [2]辽东生物产业研究所,辽宁丹东118003

出　　处：《沈阳农业大学学报》2016年第1期109-113,共5页Journal of Shenyang Agricultural University

基　　金：辽宁省教育厅科学技术研究项目(L2012472);丹东市科学技术计划项目(农业攻关及产业化)(201402)

摘　　要：为鉴定人工栽培蛹虫草致病木霉的种类,从被木霉侵染的人工栽培蛹虫草培养料中分离获得-株木霉CCBH-M1,经感染试验确定其致病性.通过对其进行形态学鉴定及ITS 序列系统发育分析,确定该木霉菌株为深绿木霉Trichoderma atroviride Karsten.通过单因素试验对该致病菌的生物学特性进行分析,结果表明： 温度、pH 值及培养料含水量对该深绿木霉生长均有显著影响（p〈0.05）,其适宜生长温度为24-32,适宜生长pH 值为5.0-7.0,培养基适宜含水量为50%-70%.可见,蛹虫草栽培过程中,在不影响蛹虫草生长的情况下,将温度、pH值和含水量三个因素分别控制在24以下、7.0-7.5 及75%-80%范围内,可有效抑制致病深绿木霉的发生.For identifying pathogenic Trichoderma species from cultivated Cordyceps militaris, strain CCBH-M1 was isolated from culture materials. The pathogenicity was determined by infection experiment. The morphological characteristics and phylogenetic analysis by ITS sequence showed that strain CCBH-M1 was Trichoderma atroviride Karsten. The optimum range of temperature, p H value and culture medium water content of T. atroviride were determined as 24-32℃, pH 5.0-7.0 and water content of 50%-70% respectively. These three factors significantly influenced the growth of strain CCBH-M1(P<0.05). To inhibit T. atroviride growth, the temperature should be controlled below 24℃, pH value between 7.0-7.5 and culture medium water content between 50-70% in cultivated process of Cordyceps militaris.

关 键 词：蛹虫草 致病木霉 鉴定 深绿木霉 生物学特性 

分 类 号：Q939[生物学—微生物学]