基于酯型生物碱含量变化选择蒸制附片  

Selection of suitable herbs for steaming Fupian based on the change of contents of ester-type alkaloids

作　　者：谭茂兰[1] 黄勤挽[1,2] 肖芳[1] 范润勇 王智磊[1] 易佳佳 

机构地区：[1]成都中医药大学药学院,中药材标准化教育部重点实验室,四川省中药资源系统研究与开发利用重点实验室-省部共建国家重点实验室培育基地,四川成都611137 [2]国家中医药管理局中药炮制技术重点研究室,四川成都611731

出　　处：《中成药》2016年第2期366-372,共7页Chinese Traditional Patent Medicine

基　　金：国家自然科学基金(81274086);四川省科技厅支撑计划(2013SZ0140);四川省中医药管理局青年基金(2010-12)

摘　　要：目的基于6种酯型生物碱含有量的变化,选择合适的蒸制附片。方法采用HPLC法,测定鲜附片、生附片、浸附片中3种双酯类生物碱（中乌头碱、乌头碱、次乌头碱）和3种单酯类生物碱（苯甲酰中乌头碱、苯甲酰乌头原碱、苯甲酰次乌头原碱）的含有量。结果蒸制过程中双酯型生物碱的含有量迅速减少,而单酯型生物碱的含有量迅速增加,然后稳定在一定水平,但随蒸制时间延长会逐渐降低。结论鲜附片更适合进行蒸制,蒸制时间以4~10 h为宜。AIM To select the suitable herbs for steaming Fupian（ Radix Aconiti lateralis preparata slice）based on the change of contents of six ester-type alkaloids. METHODS HPLC was used for determining the contents of three diester-type alkaloids（ mesaconitine,aconitine,hypaconitine） and three monoester-type alkaloids（ benzoylmesaconitine,benzoylaconine,benzoylhypaconine） in fresh,dried and soaked-cut Fupian. RESULTS The contents of diester-type alkaloids were decreased significantly,while those of monoester-type alkaloids were increased rapidly and then stabilized at a certain level. But with the extension of steaming time,the contents of monoester-type alkaloids were also decreased gradually. CONCLUSION Fresh-cut Fupian is more suitable for steaming,and the steaming time is 4-10 hours.

关 键 词：鲜附片 生附片 浸附片 蒸制 酯型生物碱 HPLC 

分 类 号：R283[医药卫生—中药学]