Ghrelin促乳腺癌细胞增殖和抑制其凋亡的作用及机制  

The impact on the expression of apoptosis related genes after MDA- MB- 231 regulated by Ghrelin of proliferated concentration

作　　者：杨蕾[1] 涂巍[1] 金光华[1] 温健[1] 范莹[1] 曲文志[1] 

机构地区：[1]中国医科大学附属第四医院乳腺外科,辽宁沈阳110032

出　　处：《现代肿瘤医学》2016年第6期854-857,共4页Journal of Modern Oncology

基　　金：辽宁省科学计划项目(编号:2013225303)

摘　　要：目的:探讨Ghrelin在促MDA-MB-231细胞增殖的浓度下,对凋亡相关基因(Bcl-2、Bax)表达的影响。方法:通过细胞培养、MTT、流式细胞检测方法探究酰基化Ghrelin对MDA-MB-231细胞系的作用,运用Western blot检测酰基化Ghrelin在促增殖浓度下对MDA-MB-231细胞系凋亡相关蛋白表达的影响。结果:MTT检测得出Ghrelin浓度在0 nmol/L~≤100 nmol/L范围内促进MDA-MB-231细胞增殖,在>100nmol/L^1 000nmol/L范围内抑制MDA-MB-231细胞增殖,且在100nmol/L时促增殖作用最强。Ghrelin浓度在100nmol/L时,流式细胞检测实验组和对照组细胞系凋亡有显著统计学差异,实验组凋亡率小于对照组。Western blot检测显示,实验组与对照组相比,Bcl-2表达增加,Bax表达下降,有统计学差异。结论:Ghrelin在一定的较低浓度范围内,促MDA-MB-231细胞增殖,并伴有Bcl-2/Bax比率升高,推测Ghrelin促MDA-MB-231细胞增殖与抗线粒体凋亡因子释放有关。Objective： To explore the effect on the gene expression related to apoptosis,such as Bcl- 2 and Bax,when MDA- MB- 231 cells are regulated by Ghrelin of proliferated concentration. Methods： We observed the n- octanoylated Ghrelin affected MDA- MB- 231 cells by cell culture,MTT and flow cytometry was used to test the alteration of related apoptosis proterin. Results： When the concentration of Ghrelin ranged from 0nmol / L - 100 nmol / L,Ghrelin promoted MDA- MB- 2 3 1 cells proliferation,however when its concentration was between 100 nmol / L and 1 000 nmol / L,Ghrelin inhibited cells proliferation,and we also found that Ghrelin had the strongest action of proliferation- promoting at 100 nmol / L. The experimental group（ 100 nmol / L Ghrelin deal with MDA- MB- 231 cells）campared with control group（ MDA- MB- 231 cells without Ghrelin） had significant statistics difference,the ratio of Bcl- 2 / Bax in experimental group was higher than control group,with significant difference. Conclusion： Lower Ghrelin concentration can promote MDA- MB- 231 cells proliferation with rised Bcl- 2 / Bax ratio,we suppose that Ghrelin promote MDA- MB- 231 cells proliferation have relationship with the release of mitochondria antiapoptotic factors.

关 键 词：GHRELIN MDA-MB-231细胞系 BCL-2 BAX 

分 类 号：R737.9[医药卫生—肿瘤]