小鼠黑色素瘤B16F10-Red细胞株的建立与生物学特性分析  

Establishment and biological characteristics of the cell line of mouse melanoma B16F10- Red

作　　者：吴伶俐[1] 孙永强[2] 王建红[3] 何向锋[3] 周玉清[3] 郑丽云[3] 赵文静[3] 陈橼[3] 李国兴[3] 施文[2] 

机构地区：[1]东南大学附属中大医院检验科,江苏南京210009 [2]南通大学第二附属医院普外科,江苏南通226001 [3]南通大学附属南通市肿瘤医院内科,江苏南通226361

出　　处：《现代肿瘤医学》2016年第6期861-864,共4页Journal of Modern Oncology

基　　金：南通市卫生局青年基金项目(编号:WQ2014012);南通市应用研究计划项目(编号:BK2012067);南通市社会事业科技创新与示范计划项目(编号:HS2013031)

摘　　要：目的:构建表达香菇珊瑚红色荧光蛋白(discosomasp red fluorescent protein,DsRed)的小鼠黑色素瘤B16F10-Red细胞株,并检测其生物学特性。方法:用Gen EscortTMⅡ转染试剂将p DsRed质粒导入小鼠黑色素瘤B16F10细胞,G418加压培养联合极限稀释法建立稳定、高水平表达DsRed的单克隆细胞系。FCM检测B16F10和B16F10-Red细胞的细胞周期。比较B16F10-Red和B16F10细胞的克隆球形成能力和小鼠体内致瘤能力。结果:稳定表达DsRed的小鼠黑色素瘤B16F10-Red细胞株基本保持了其亲代细胞的特征,能在C57BL/6小鼠腹部皮下形成肿瘤并继续生长和转移。结论:B16F10-Red细胞株构建成功,其移植瘤模型成瘤率和转移情况同B16F10肿瘤相比无明显差别。Objective： To build expression discosomasp red fluorescent protein mouse melanoma tumor B16F10-Red cell lines and detect its biological characteristics. Methods： DsRed plasmids were introduced into mouse melanoma B16F10 cells transfected with Gen EscortTMⅡ reagents,G418 pressure cultured the joint limiting dilution method to establish a stable,high- level expression of RFP monoclonal cell lines. FCM was used to detect B16F10 and B16F10- Red cell cycle. B16F10- Red cells colony forming ability was compared with B16F10 cells and mice in vivo tumorigenicity force. Results： The stable expression of RFP mouse melanoma cell line B16F10- Red substantially maintained its parental cell characteristics,can form tumors in C57 BL /6 mice subcutaneous abdominal and continue growth and metastasis. Conclution： The B16F10- Red cell lines were successfully constructed,its rate of tumor formation and metastasis xenograft model was no significant difference compared with B16F10 tumor.

关 键 词：黑色素瘤 B16F10 红色荧光蛋白 肿瘤移植 

分 类 号：R739.5[医药卫生—肿瘤]