水飞蓟宾抑制乳腺癌细胞增殖和促进其凋亡的作用及机制  

The role of silybin in inhibiting proliferation and promoting apoptosis of breast cancer cells

作　　者：周大勇[1] 还勇为[1] 

机构地区：[1]解放军第82医院普外科,江苏淮安223001

出　　处：《现代肿瘤医学》2016年第6期882-885,共4页Journal of Modern Oncology

摘　　要：目的:探讨水飞蓟宾抑制乳腺癌细胞增殖和促进其凋亡的作用及机制。方法:MCF7细胞分为对照组和SIL组(处理浓度分别为10、20、40mg/L),各组细胞分别处理24、48、72h。MTT法检测不同浓度SIL对乳腺癌MCF7存活率的影响,TUNEL法(DNA断裂的原位末端标记法)检测各组细胞凋亡率,caspase-Glo83/7定量试剂盒测定细胞caspase3/7的表达情况,Western blot法检测PUMA蛋白表达水平。结果:MTT结果显示SIL对MCF7细胞增殖有抑制作用(P<0.01),其中用40mg/L SIL处理MCF7细胞72h时抑制效果最明显,结果呈药物浓度时间依赖效应。TUNEL法显示(48h)SIL可以显著增加MCF7细胞的凋亡率(P<0.01)。caspase3/7结果示SIL可以使caspase3/7活性显著升高(P<0.01)。Western blot示SIL可以诱导PUMA蛋白的表达升高。结论:水飞蓟宾能抑制乳腺癌细胞增殖和促进其凋亡,其作用机制之一可能是诱导PUMA蛋白表达。Objective： To investigate the role of silybin in inhibiting proliferation and promoting apoptosis of breast cancer MCF7 cells. Methods： The cells were divided into control group and SIL groups（ 10,20,40 mg / L）,each group was treated for 24,48 and 72 h. MTT method was used to detect the survival rate of cells. TUNEL was used to detect the apoptotic rate of cells. Special kits were used to detect the activity of caspase3 /7. Western bolt method was used to detect the expression of PUMA. Results： The MTT results show that SIL could inhibit the proliferation of MCF7 cells on dose and time- depend manner,the effect of 40 mg / L SIL（ 72h） was the most obvious. After being treated with SIL（ 48h）,the apoptotic rate of cells and the activity of caspase3 /7 increased significantly. In addition,PUMA protein increased significantly in SIL treated group. Conclusion： Silybin can inhibit proliferation and promote apoptosis of breast cancer MCF7 cells. PUMA may play a role in the apoptosis of breast cancer MCF7 cells induced by silybin.

关 键 词：水飞蓟宾 PUMA 乳腺癌 MCF7细胞 

分 类 号：R73-3[医药卫生—肿瘤] R737.9[医药卫生—临床医学]