3种鳜鱼生长与摄食量、胃蛋白酶活性和胃蛋白酶原基因表达相关分析  

Comparison of growth,food intake,pepsin activity and pepsinogen genes expression among Siniperca species

作　　者：李传阳[1] 许淼洋[1] THAMMARATSUNTORN Jeerawat 赵金良[1] 钱叶洲 吴超 钱德 

机构地区：[1]上海海洋大学农业部淡水水产种质资源重点实验室,上海201306 [2]安徽省池州市秋浦特种水产开发有限公司,安徽池州247104

出　　处：《上海海洋大学学报》2016年第1期1-7,共7页Journal of Shanghai Ocean University

基　　金：上海市科委基础研究重点项目(09JC1406900);水产动物遗传育种中心上海市协同创新中心(ZF1206);池州市秋浦特种水产开发有限公司专项资金(2012A01)

摘　　要：为了解不同种类鳜鱼生长差异的生理基础与机制,比较研究了鳜(Siniperca chuatsi)、斑鳜(Siniperca.scherzeri)和杂交鳜(斑鳜♀×鳜♂)幼鱼30 d内的生长速率与日摄食量、胃蛋白酶活性和胃蛋白酶原(PGA1、PGA2、PGC)基因相对表达量间的关系。结果表明,鳜、杂交鳜和斑鳜日增重率分别为(1.171±0.180)g/d、(1.019±0.104)g/d和(0.433±0.078)g/d,生长速率快慢次序是鳜>杂交鳜>斑鳜。鳜、杂交鳜和斑鳜平均日摄食量分别为(6.1±0.31)g/d、(5.26±0.33)g/d、(4.24±0.23)g/d。胃蛋白酶比活力最高部位是胃,实验前期,3种鳜鱼胃蛋白酶比活力差异不显著;后期,鳜、杂交鳜和斑鳜胃蛋白酶比活力分别为(347.8±13.3)U/g、(303.4±12.1)U/g和(272.1±10.9)U/g。胃蛋白酶原基因表达量随生长阶段缓慢增加,PGC∶PGA1∶PGA2基因相对表达量比例是1.1∶1.0∶0.7;鳜胃蛋白酶原基因的相对表达量均为最高,杂交鳜相对表达量略高于斑鳜(P<0.05)。结果表明,3种鳜鱼的生长速率快慢与它们的摄食量大小之间呈正相关,摄食量大小与其消化能力间也具有相对应的关系。To further understand the physiological mechanism of growth of different mandarin fish,the relationship between growth rates and daily food intake,pepsin activities,relative expression of pepsinogen PGA1,PGA2,PGC in stomach of juveniles of S. chuatsi,S. scherzeri and their hybrids（ S. scherzeri ♀ × S.chuatsi ♂ F1） were compared and analyzed during 30 days＇ experiment. Results showed that average growth weight（ AGW） of three species was（ 1. 171 ± 0. 180） g / d of S. chuatsi,（ 1. 019 ± 0. 104） g / d of hybrids,（ 0. 433 ± 0. 078） g /d of S. scherzeri,respectively,and their growth rates ranked as S. chuatsi F1 S.scherzeri. Daily food intake of the three species was（ 6. 1 ± 0. 31） g / d of S. chuatsi,（ 4. 24 ± 0. 23） g / d of S. scherzeri and（ 5. 26 ± 0. 33） g / d of hybrids,respectively,S. chuatsi was higher than hybrids and S.scherzeri. The maximum specific pepsin activity was found in stomach,specific pepsin of the three species was（ 347. 8 ± 13. 3） U/g of S. chuatsi,（ 272. 1 ± 10. 9） U/g of S. scherzeri（ 303. 4 ± 12. 1） U/g of hybrids during later periods. Among the three species,pepsin activities ranked as S. chuatsi F1 S. scherzeri.Gastric PGs mRNA expression of three species all increased during the experiment. Proportion of PGC ∶PGA1∶ PGA2 was 1. 1 ∶ 1. 0 ∶ 0. 7. The highest expression level of PGs was observed in S. chuatsi,PGs expression of F1 was slightly higher than S. scherzeri（ P〈0. 05）. Growth rate was in direct correlation with amount of food intake of the three Siniperca species,and there was a corresponding relationship between amount of food intake and digestive capacity.

关 键 词：鳜鱼 生长 日摄食量 胃蛋白酶 活性 基因表达 

分 类 号：S917[农业科学—水产科学]