固相萃取-高效液相色谱法测定水产品中甲氧苄啶残留量  

Determination of trimethoprim in aquatic products by SPE-HPLC

作　　者：孙玉慧[1] 李天宝[1] 范志超[1] 李文斌 

机构地区：[1]江门出入境检验检疫局,广东江门529000 [2]北京迪马科技有限公司,北京100029

出　　处：《中国测试》2016年第2期56-58,共3页China Measurement & Test

基　　金：国家质检总局科技计划项目(2015K063)

摘　　要：建立固相萃取一高效液相色谱法检测水产品中甲氧苄啶残留量的分析方法。试样用15mL5％乙酸水一甲醇（70：30，v：v）、1mL乙酸铅（200g／L）、7mL三氯甲烷混合液提取，提取液经ProElutPXC固相萃取柱净化，高效液相色谱仪对水产品中甲氧苄啶残留量进行测定。在0．02～0．20mg／L范围内甲氧苄啶的质量浓度与色谱峰面积呈良好的线性关系，相关系数为0．9993，方法的检出限（S／N=3）为0．02mg／L，平均回收率为83．4％～87．0％，相对标准偏差（RSD）为3．9％～4．9％。该方法适合水产品中甲氧苄啶残留量的Et常监测。A method for determining the based on solid-phase extraction （SPE） trimethoprim content in aquatic and high performance liquid products was developed chromatography （HPLC）. Samples were extracted with the mixture of 5% acetic aeid-methanol（15mL, v：v=70：30）, lead acetate（1 mL, 200g/L） and triehlormethane（7mL）and then purified with a solid-phase extraction column ProElut PXC. The extract liquid was determined by HPLC. The concentration of the trimethoprim was linear with the peak area in the range of 0.02-0.20 mg/L. The correlation coefficient was 0.999 3, the limit was 0.02 mg/L, the average recovery rate was 83.4%-87.0%, and the relative standard deviation （RSD） was 3.9%-4.9%. The method can be applied to determine the trimethoprim content in aquatic products.

关 键 词：固相萃取 高效液相色谱 水产品 甲氧苄啶 

分 类 号：TS218[轻工技术与工程—粮食、油脂及植物蛋白工程]