高效液相非接触电导法测定阿咖酚散3种有效成分的含量  

Determination of 3 Effective Components in Akafen Powder with Contactless Conductivity Detection by HPLC

作　　者：肖羽[1,2] 陈安丽[1,2] 尹玮璐[1] 吴静芳[1] 陈缵光[2] 

机构地区：[1]广州市药品检验所,广东广州510160 [2]中山大学药学院,广东广州510006

出　　处：《今日药学》2016年第1期33-35,共3页Pharmacy Today

摘　　要：目的建立高效液相非接触电导检测法快速测定阿咖酚散中3种有效成分含量的新方法。方法色谱系统采用ODS反相柱,流动相：甲醇-水-乙酸（35∶60∶5）,流速：1.0 mL/min,柱温：35℃,进样量：10μL,用非接触电导检测器对阿司匹林、对乙酰氨基酚、咖啡因进行分离和检测。结果阿司匹林线性范围为36.00-359.96μg/mL（r=0.9866）,平均回收率（n=9）为99.6%;对乙酰氨基酚线性范围为19.97-199.32μg/mL（r=0.9863）,平均回收率（n=9）为100.5%;咖啡因线性范围为4.9-49.04μg/mL（r=0.9680）,平均回收率（n=9）为100.1%。结论方法简便、灵敏、重复性好,适用于阿咖酚散中3种有效成分的含量测定,结果满意。OBJECTIVE To establish a new method for the rapid determination of three effective components in Akafen powder by HPLC determination with contactless conductivity detection. METHODS HPLC method was carried on a column of ODS at column temperature of 35 ℃,with the mobile phase of methanol-water（ 40 ∶60）,as mobile phase at flow rate of 1. 0 mL / min,separation and detection of aspirin,paracetamol,and caffeine by contactless conductivity detection.RESULTS The linear range was 36.00-359.96 μg / mL（ r = 0.986 6）,and the average recoveries（ n = 9） was 99.6% for aspirin,19.97-199.32 μg / mL（ r = 0. 986 3） and the average recoveries（ n = 9） was 100.5% for paracetamol,4.9-49.04 μg /mL（ r = 0.968 0） and the average recoveries（ n = 9） was100.1%for caffeine. CONCLUSION The method was convenient,rapid and reproducible,and it could be used for rapid determination of Akafen powder with satisfactory result.

关 键 词：高效液相 非接触电导检测 阿咖酚散 阿司匹林 对乙酰氨基酚 咖啡因 

分 类 号：R27[医药卫生—中医学]