上海松江地区汉族人群NAD（P）H氧化酶C242T基因多态性与高脂血症及脑梗死的相关性研究  

Association of NAD（P）H oxidase C242T gene polymorphism with lipid level and lipid metabolismof patients with cerebral infarction from Shongjiang Shanghai

作　　者：蒋萍[1] 李丽敏[1] 顾彬[1] 于芳苹[1] 潘晓春[1] 杨志文[1] 赵迎春[1] 

机构地区：[1]南京医科大学附属上海松江中心医院神经内科,上海201699

出　　处：《中华神经医学杂志》2016年第2期164-171,共8页Chinese Journal of Neuromedicine

基　　金：上海市松江区卫生局医学领先合作项目(2014LX02) Leading Medical Cooperation Projects of Sanitary Bureau of Shongjiang Shanghai

摘　　要：目的探讨上海地区汉族人群还原型烟酰胺腺嘌呤二核苷酸磷酸[NAD（P）H]氧化酶C242T基因多态性与高脂血症及脑梗死的相关性。方法采用病例一对照研究设计，联合聚合酶链反应．限制性片段长度多态性分析（PCR-RFLP）、基因测序技术、分光光度技术、酶联免疫吸附技术，对上海松江地区汉族人群中207例高脂血症患者（自2014年3月至2015年3月在上海松江中心医院健康体检中心体检的非心脑血管病的高脂血症患者）、185例脑梗死患者（自2014年3月至2015年3月在上海松江中心医院神经内科住院的脑梗死患者）和183例对照者（自2014年3月至2015年3月在上海松江中心医院健康体检中心体检的无脑血管病病史、无高脂血症病史、年龄相匹配的健康中老年人1的C242T基因多态性位点进行基因型、等位基因频率比较．以及对高脂血症组与对照组间，脑梗死组与对照组间，各基因型组间的临床资料、脂蛋白氧化水平、NAD（P）H氧化酶活性等指标进行相关性分析。结果高脂血症组低密度脂蛋白水平、氧化低密度脂蛋白水平、丙二醛、NAD（P）H氧化酶活性与对照组比较差异均无统计学意义（P〉0．05），基因型频率及等位基因频率与对照组相比差异均无统计学意义（P〉O．05）。脑梗死组氧化高密度脂蛋白、丙二醛、NAD（P）H氧化酶活性水平明显高于对照组，CT＋TT基因型频率及T等位基因频率明显高于对照组，差异均有统计学意义（P〈0．05）。CT＋TT基因型组在甘油三酯、胆固醇、高密度脂蛋白水平和CC基因型组比较差异均无统计学意义（P〉0．05）；而CT＋TT基因型组氧化高密度脂蛋白、氧化低密度脂蛋A及丙二醛水平、NAD（P）H氧化酶活性均明显高于CC基因型组，差异均有统计学意义（P〈0．05）。Logistic回归分析发现．吸烟、高血压、脂蛋白a水平、氧化高密度脂�Objective To explore the relations ofnicotinamide adenine dinucleotide phosphate （NAD [P]H） oxidase p22phox C242T gene polymorphism with hyperlipidemia and cerebral infarction. Methods A case-control association study containing 207 hyperlipidemia patients （collected from Physical Examination Center from March 2014 to March 2015）, 185 cerebral infarction patients （collected from Department of Neurology from March 2014 to March 2015） and 183 controls （collectedfrom Physical Examination Center from March 2014 to March 2015） was carried out. The genetypes and allele frequency were compared; the clinical data, lipoprotein oxidation level and NAD （P）H oxidase activity were performed correlation analysis between different patient groups and different genetypes. Results There were no differences in low-density lipoprotein （LDL）, oxidized LDL and malondialdehyde levels, NAD （P）H oxidase activity, and frequencies of CT＋TT genotype and T allelic gene between patients with hyperlipidemia and controls （P〉0.05）. Group of cerebral infarction had significantly higher serum levels of oxidized high-density lipoprotein （HDL） and malondialdehyde, and NAD（P）H oxidase activity than the controls （P〈0.05）; the frequencies of CT＋TT genotype and T allelic gene in patients with cerebral infarction were significantly higher than those in the controls （P〈0.05）. There was no difference between CC genotype patients and CT＋TT genotype patients in the serum levels of triglyceride, cholesterol and HDL （P〉0.05）, but CC genotype patients had significantly higher serum levels of oxidized HDL, oxidized LDL and malondialdehyde, and NADPH oxidase activity than CT＋TT genetype patients （P〈0.05）. Logistic regression analysis indicated that smoking, hypertension, serum levels of lipoprotein （a）, oxidized HDL and malondialdehyde, NADPH oxidase activity and T allelic gene （OR=0.299, 95%CI： 0.102-1.879, P=-0.028） were independent risk factors for cerebral infarction. Conclusi

关 键 词：还原型烟酰胺腺嘌呤二核苷酸磷酸氧化酶 C242T基因多态性 脂蛋白氧化 高脂血症 脑梗死 

分 类 号：R743.3[医药卫生—神经病学与精神病学] R589.2[医药卫生—临床医学]