Lipocalin-2过表达对人肺癌A549细胞增殖、凋亡及侵袭力的影响  

Effects of overexpression of Lipocalin-2 on proliferation,apoptosis and invasiveness in human lung cancer A549 cells in vitro

作　　者：马小刚[1] 杨晓光[1] 任敬[1] 赵峥[1] 

机构地区：[1]河北省邯郸市中心医院胸外科,056001

出　　处：《河北医药》2016年第1期27-30,共4页Hebei Medical Journal

摘　　要：目的采用基因过表达方法上调人肺癌A549细胞Lipocalin-2表达,观察Lipocalin-2对细胞增殖、凋亡、细胞周期调控及侵袭力的影响。方法构建Lipocalin-2过表达慢病毒载体,转染人肺癌A549细胞。Real-time PCR和Western blot检测转染后人肺癌A549细胞中Lipocalin-2表达水平。MTT实验检测细胞增殖。流式细胞术检测细胞周期及凋亡情况。Transwell小室实验检测细胞侵袭力。结果与阴性对照组和空载体对照组比较,转染组Lipocalin-2 mRNA和蛋白表达水平均显著增加,差异有统计学意义(P<0.05)。Lipocalin-2过表达慢病毒载体转染后,细胞增殖率和侵袭力显著增加,同时细胞凋亡率及G0/G1期细胞比例显著降低,S期细胞比例显著提高,差异有统计学意义(P<0.05)。结论 Lipocalin-2过表达通过调控人肺癌A549细胞周期、抑制凋亡促进细胞的增殖,还明显提高细胞的侵袭力。Objective To investigatethe effects of overexpression of Lipocalin-2 on cell proliferation, cell apoptosis,cell cycle regulation and invasiveness in human lung cancer A549 cells in vitro through up - regulating the expression levels of Lipocalin-2. Methods Lipocalin-2 gene coding region was cloned into lentivirns vector, then lentivirus particles were transfected into A549 cells to up-regulate the expression levels of Lipocalin-2 gene. The expression levels of Lipocalin-2 mRNA and protein were detected by Real-time PCR and Western Blot, respecttvely. The cell proliferation was determined by MTT assay and cell apoptosis and cell cycle were detected by flow cytometry. The cell invasiveness was measured by Transwell test. Results The expression levels of Lipocalin-2 mRNA and protein were ihcreased significantly after the transfection, as compared with those in negative control group or empty vector control group （ P 〈 0.05 ）. Moreover the cell proliferation rate and invasiveness were significantly increased,however, the cell apoptosis rate and ratio of G0/G1 were obviously decreased, but the cell proportion at stage S was significantly increased （ P 〈 0.05 ）. Conclusion The overexpression of Lipocalin-2 can promote proliferation of A549 cells through regulating cell cycle and inhibiting Cell apoptosis.

关 键 词：肺肿瘤 Lipocalin-2 A549细胞 增殖 凋亡 侵袭 

分 类 号：R734.2[医药卫生—肿瘤]