基于不同缓冲体系的纤维素酶活力及其稳定性研究  

Analysis of activity and stability of cellulase based on different buffer systems

作　　者：李倩[1] 隋博 姚继明[1] 

机构地区：[1]河北科技大学纺织服装学院,河北石家庄050018 [2]大连九信生物化工科技有限公司,辽宁大连116000

出　　处：《染整技术》2016年第2期4-7,22,共5页Textile Dyeing and Finishing Journal

基　　金：河北省自然科学基金资助项目(B2013208082);河北科技大学五大平台开放基金(FZ)

摘　　要：采用柠檬酸、醋酸和磷酸3种不同的缓冲体系、分别在pH为4.8、6.0,温度为40℃、50℃、60℃的条件下,测试纤维素酶的滤纸酶活力,分析酶活数值的高低和稳定性。结果表明,在pH=4.8的条件下,醋酸缓冲体系中的酶活数值最高,数值稳定性则是柠檬酸缓冲体系最好;在pH=6.0的条件下,无论酶活数值还是稳定性都是磷酸缓冲体系高于醋酸和柠檬酸缓冲体系。Filter paper activity（FPA） of cellulase was measured using three buffer solutions of citric acid, acetic acid and phosphoric acid, under the conditions of pH （4.8, 6.0） and temperature （40℃, 50℃, 60℃ ）. FPA values of cellulase and their stability were also analysed. The results show that in case of pH=4.8, the highest value was obtained by acetic acid buffer solution, while highest stability was obtained by citric acid buffer system. Both of the highest values and stability were obtained by phosphoric acid buffer solution at pH=6.0.

关 键 词：纤维素酶 酶活性 缓冲体系 磷酸 醋酸 柠檬酸 

分 类 号：TQ426.97[化学工程]