光纤和显微拉曼光谱结合化学计量学鉴别胃癌组织  

Fiber-optic and microscopic Raman spectroscopy combined with chemometrics for discrimination of gastric cancer tissue

作　　者：易伟松[1] 钱辉跃[1] 江厚敏[2] 

机构地区：[1]华中农业大学理学院,湖北武汉430070 [2]武汉市黄陂区人民医院,湖北武汉430300

出　　处：《中国医学物理学杂志》2016年第2期134-140,共7页Chinese Journal of Medical Physics

基　　金：湖北省自然科学基金(2014CFB476);中央高校基本科研业务费专项资金(2662015PY157;529020900206178);出国留学基金(201406765056)

摘　　要：目的:胃癌具有高发生率、高死亡率的典型特征,是严重影响人类健康的重大疾病。拉曼光谱因其无损、快速、高灵敏性、高特异性,且易与各种探针有机结合可以实现原位、活体和实时检测等特征,极具医学临床应用潜力。研究光纤和显微拉曼光谱结合化学计量学方法鉴别胃癌组织的可行性及科学性。方法:分别利用光纤拉曼和显微拉曼光谱仪获取胃癌组织及正常组织的拉曼光谱,经过平滑、基线扣除及归一化等预处理后,结合化学计量学方法,以指纹范围(800~1700 cm^(-1))的全波段拉曼光谱作为输入变量,进行主成分分析和聚类分析,同时以病理学诊断结果作为依据,从正确率、灵敏度和特异性等指标评价前述鉴别效果。结果:正常组织和癌变组织的拉曼光谱存在显著差异,拉曼光谱结合化学计量学方法鉴别诊断效果良好,显微拉曼光谱聚类分析的正确率、灵敏度和特异性分别为90.0%、91.9%和88.9%;光纤拉曼光谱聚类分析的正确率、灵敏度和特异性分别为87.8%、86.7%和88.9%。结论:拉曼光谱结合化学计量学能够鉴别胃癌组织,具有从原理到临床应用的一致性。Objective Gastric cancer is a major disease that seriously affects human health, with typical characteristics of high incidence and mortality. Raman spectroscopy has great potential for clinical application because it is non-destructive, rapid, with high sensitivity and specificity, and it can achieve the detection and diagnosis in vivo, in situ and real-time by easily integrating with various medical probes. To analyze the feasibility and scientificity of fiber-optic and microscopic Raman spectroscopy combined with chemometrics for the diagnosis of gastric cancer tissue. Methods The Raman spectrums of gastric cancer tissue and normal tissue were respectively obtained by fiber-optic and microscopic Raman spectroscopy. After the preprocess of smoothing, baseline elimination and normalization, combined with chemometrics, the Raman spectrums at the fingerprint range from 800 to 1700 cm^-1 were taken as input variables for the principal component analysis and cluster analysis. Based on the pathological diagnostic result, the diagnostic result of Raman spectroscopy combined with chemometrics was evaluated by analyzing the accuracy, sensitivity, specificity and so on. Results Significant differences were found between the Raman spectrum of gastric cancer tissue and that of normal tissue. The discrimination results of the Raman spectroscopy combined with chemometrics were satisfactory. The accuracy, sensitivity, specificity of microscopic Raman spectroscopy were respectively 90.0%, 91.9% and 88.9%, while those of fiber-optic Raman spectroscopy were respectively 87.8%, 86.7% and 88.9%. Conclusion Raman spectroscopy combined with chemometrics has a satisfactory result in the diagnosis of gastric cancer tissue, achieving the consistency of principle and clinical application.

关 键 词：光纤拉曼光谱 显微拉曼光谱 化学计量学 鉴别 胃癌 

分 类 号：R44[医药卫生—诊断学] R735.2[医药卫生—临床医学]