未成年人群网织红细胞参数参考区间的初步建立  

Establishment of the reference intervals of 7 reticulocytes parameters among healthy juveniles

作　　者：罗小娟[1] 曹科[1] 马东礼[1] 周晨阳[1] 郎家庆[1] 谢辉辉[1] 王丹[1] 陈运生[1] 杨斌让[2] 

机构地区：[1]广东省深圳市儿童医院检验科,518038 [2]广东省深圳市儿童医院儿童保健科,518038

出　　处：《检验医学与临床》2016年第4期466-468,471,共4页Laboratory Medicine and Clinic

基　　金：深圳市科创委资助项目(JCYJ20140416141331504)

摘　　要：目的建立未成年人群Sysmex-XN3000全自动血细胞分析流水线7项网织红细胞参数的参考区间。方法参照美国临床和实验室标准化协会（CLSI）C28-A3文件推荐方法,检测423例未成年人群标本,自建适用于该实验室的参考区间。结果 （1）346例健康者网织红细胞参数结果,男187例,女159例,1个月至1岁组38例,~3岁组72例,~6岁组54例,~12岁组124例,~18岁组58例。（2）~3岁组和~18岁组的女性RET%高于男性,差异有统计学意义（P〈0.05）,其余项目同年龄男、女性组间差异无统计学意义（P〉0.05）;RET#和RET%不同年龄组间差异无统计学意义（P〉0.05）,其余项目不同年龄组间差异有统计学意义（P〈0.05）,前2组（1个月至1岁组与~3岁组）和后3组（~6岁组、~12岁组、~18岁组）的LFR%、MFR%、HFR%、IRF%差异无统计学意义（P〉0.05）,前3组（1个月至1岁组、~3岁组、~6岁组）和后2组（~6岁组与~12岁组）的（RET-He）结果差异无统计学意义（P〉0.05）,其余各组间差异有统计学意义（P〈0.05）。（3）RET%和RET#检测结果与年龄无相关性,MFR%、HFR%、IRF%检测结果与年龄呈负相关,LFR%和RET-He检测结果与年龄呈正相关。（4）RET#和RET%在1个月至18岁的参考区间分别为（30.1~103.6）109/L和（0.65~2.41）%;LFR%、MFR%、HFR%、IRF%在1个月至3岁的参考区间分别为（84.2~98.1）%、（2.0~13.2）%、（0.0~3.2）%、（2.2~16.1）%,3岁~18岁的参考区间分别为（89.5~98.6）%、（1.4~9.5）%、（0.0~1.3）%、（1.4~10.5）%;RET-He在1个月至12岁和12~18岁的参考区间分别为（28.3~34.6）pg和（28.5~35.4）pg。结论该实验室建立的健康未成年人群7项网织红细胞参数的参考区间,适合该地区人群,值得临床推广应用。Objective To establish the reference intervals of 7reticulocytes parameters on Sysmex-XN3000 in healthy juveniles.Methods According to the methods recommended by American Journal of Clinical and Laboratory Standards Institute（CLSI）C28-A3,the 7reticulocytes indexs werw tested in specimens of 423 juveniles to establish a proper biology reference interval.Results（1）A total of 346 qualified specimens from juveniles were recruited,including 187 males and 159 females,with 38,72,54,124 and 58subjects for 1months to 1years group,-3years group,-6years group,-12 years group and-18 years group.（2）The reticulocytes parameters showed no significant difference between boys and girls（P〉0.05）,but the RET% of-3years and-18 years girls were significantly higher than that in boys（P〉0.05）.The reticulocytes parameters show significant differences in different age groups except RET# and RET%（P〈0.05）,but LFR%,MFR%,MFR%,IRF% showed no significant difference in-6years group,-12 years group and-18 years group,between 1months to 1years group and-3years group.The RET-He showed no significant difference in 1 months to 1years group,-3years group and-6years group,between-6years group and-12 years group（P〈0.05）.（3）The RET% and RET# has no correlation with age,MFR%,HFR% and IRF% were decreased with aging,but LFR and RET-He were increased with aging.（4）Reference intervals of RET# and RET% were（30.1-103.6）10~9/L and（0.65-2.41）%in 1months to 18years;reference intervals of LFR%,MFR%,HFR% and IRF% were（84.2-98.1）%,（2.0-13.2）%,（0.0-3.2）% and（2.2-16.1）%in 1months to 3years;（89.5-98.6）%,（1.4-9.5）%,（0.0-1.3）% and（1.4-10.5）%in 3years to 18years;reference intervals of RET-He were（28.3-34.6）pg in 1 months to 12years;（28.5-35.4）pg in 12 years to 18 years.Conclusion The method of investigating the reference intervals to satisfy the clinical requirement deserves to be generalization and application.The reference intervals of 7reticulocytes parameters among healthy juven

关 键 词：网织红细胞计数 健康儿童 青少年 参考区间 

分 类 号：R556.3[医药卫生—血液循环系统疾病]