氯化锂在细菌性化脓性角膜炎中的调控  

The regulatory role of LiCl in microbial suppurative keratitis

作　　者：陈康[1,2] 傅强[1] 易冰[1] 罗燕香[1] 孙世珺[1] 张秀明[1] 

机构地区：[1]中山大学附属中山医院检验医学中心,广东中山528403 [2]南方医科大学,广东广州510515

出　　处：《国际检验医学杂志》2016年第4期478-479,481,共3页International Journal of Laboratory Medicine

基　　金：国家自然科学基金青年基金项目(81401645);广东省医学科研基金(B2014447);中山市科技计划项目(2014A17C098)

摘　　要：目的探讨氯化锂(LiCl)在细菌性化脓性角膜炎中发挥的作用及其调控机制。方法 Western-blot检测LiCl应用的效果后,结膜下注射LiCl观察疾病进展;实时荧光定量PCR检测炎性因子的表达;TUNEL和流式细胞术检测细胞凋亡。结果 LiCl延缓角膜炎的疾病进展;随后实验发现LiCl促进抑炎因子分泌抑制促炎因子的分泌;促进角膜浸润细胞的凋亡。结论 LiCl通过调节炎性因子促进细胞凋亡,抑制角膜炎症反应,在铜绿假单胞菌角膜炎中发挥角膜保护作用。Objective To explore the role of LiCl in microbial suppurative keratitis.Methods Western-blot assay was used to detect the efficacy of LiCl.Inflammatory cytokine levels were examined using Real-time PCR.Cell apoptosis was detected by using TUNEL and flow cytometry assays.Results LiCl promoted corneal resistance to PA infection.Real-time PCR data showed that LiCl enhanced IL-10 expression,but suppressed TNF-expression.TUNEL and flow cytometry data showed that LiCl promoted the apoptosis of infiltrating cells.Conclusion LiCl promoted host resistance against microbial suppurative keratitis,via regulating inflammatory cytokine expression and cell apoptosis.

关 键 词：细菌性角膜炎 炎症反应 细胞凋亡 

分 类 号：R772.21[医药卫生—眼科] R440[医药卫生—临床医学]