王氏连朴饮对脾胃湿热胃癌前病变大鼠胃黏膜IL-6、NF-κb表达的影响  

Effect of Wang's lianpo decoction on IL-6 and NF-κB expressions of stomach mucosa in rat with precancerous lesions of gastric cancer of spleen stomach damp-heat syndrome

作　　者：黄琴[1] 魏瑄[1] 王和生[1] 王俊霞[1] 谭芸[1] 左海潮[1] 黄伟[1] 

机构地区：[1]贵阳中医学院

出　　处：《山西中医》2016年第2期55-57,共3页Shanxi Journal of Traditional Chinese Medicine

基　　金：贵州省中医药管理局课题(QZYY2013-107)

摘　　要：目的:观察王氏连朴饮对脾胃湿热胃癌前病变大鼠胃黏膜IL-6、NF-κb表达的影响,探讨该方逆转胃癌前病变的可能作用机制。方法:将SD大鼠85只,随机分为正常对照组、模型组、西药对照组(维甲酸,40 mg/kg)、中药小、中、大剂量组(5.43、10.85、21.70 g·kg^(-1)),共6组。除正常组饲以普通饲料外,其余5组均先以"湿热环境加高脂高糖饮食和白酒综合法"复制脾胃湿热证模型,共15日;再以"灌服热盐水、2%水杨酸,饮用脱氧胆酸钠+饥饱失常"综合法复制胃癌前病变模型,共12周。连续灌胃给药4周,观察该方对脾胃湿热胃癌前病变大鼠胃黏膜IL-6、NF-κb表达的影响及病理形态的变化。结果:模型组IL-6、NF-κb升高,与正常对照组比较,有显著性差异(P<0.01)。中药大、中、小剂量组IL-6、NF-κb表达有一定程度下调,与模型组比较,具有显著性差异(P<0.01),尤以西药组和高剂量组作用最明显。结论:王氏连朴饮可能通过下调脾胃湿热胃癌前病变大鼠胃黏膜IL-6、NF-κb水平的表达,一定程度减少胃黏膜的炎症反应,调节其胃黏膜细胞增殖与凋亡的失衡,进而达到阻断胃癌前病变的目的。Objective： To observe the effect of Wang＇s lianpo decoction on IL-6 and NF-κB expressions of stomach mucosa in rat with precancerous lesions of gastric cancer （PLGC） of spleen stomach damp - heat syndrome, and to research the possible mechanism of reversing PLGC with the formula. Methods ：85 SD rats were randomly divided into 6 groups, i. e. normal control group, model group, western medicine control group （tretinoin, 40 g/ kg）, Chinese medicine groups of high, medium-and low-dose （21.70,10. 85,5.43g·kgl）. Except the normal control group fed with ordinary feed, 5 rest groups were all made into the models of spleen stomach damp- heat syndrome by combined method of damp-heat environment ,high fat and sugar diet as well as white spirit in 15 days ;then into the PLGC model by combined method of hot brine gavage,2% salicylic acid, sodium deoxycholate and irregular hunger and satiety in 12 weeks. After continuous gavage administration for 4 weeks, the IL-6 and NF- KB expressions of stomach mucosa and pathomorphological changes of rats were observed. Results- The IL-6 and NF-KB expressions of model group was significantly increased;compared with the normal control group , there was a significant difference（ P 〈 0.01 ）. The IL-6 and NF-KB expression of Chinese medicine groups of high-, medium-, low-dose were lowered to a certain degree; compared with the model group, there was a significant difference （P 〈0. 01 ） ; especially, the effects on western medicine group and Chinese medicine groups of high-dose were most obvious. Conclusion： Through lowering IL-6 and NF-κB level expressions of stomach mucosa in rat with PLGC of spleen stomach damp-heat syndrome, Wang＇s lianpo decoction may reduce the inflammation on the gastric mucosa to a certain extent , and adjust the imbalance between proliferation and apoptosis of gastric mucosa ceils , which achieves thus the purpose of blocking PLGC.

关 键 词：胃癌前病变 脾胃湿热证 王氏连朴饮 IL-6 NF-ΚB 大鼠 实验研究 

分 类 号：R735.2[医药卫生—肿瘤]