基质金属蛋白酶-9基因R668Q多态性与动脉粥样硬化性脑梗死初发及复发相关性研究  

Study on correlation between matrix metalloproteinase-9 gene R668Q polymorphism and atherosclerotic cerebral infarction for the first time onset or recurrence

作　　者：喻明[1] 李凤[1] 李琳琳[1] 陈秋[1] 聂本刚[1] 

机构地区：[1]遂宁市中心医院神经内科,629000

出　　处：《临床神经病学杂志》2016年第1期11-14,共4页Journal of Clinical Neurology

基　　金：四川省遂宁市科知局项目(2014015)

摘　　要：目的研究基质金属蛋白酶-9（MMP-9）基因R668Q多态性与动脉粥样硬化性脑梗死初发及复发的相关性。方法采用聚合酶链反应一限制性片段长度多态性（PCR—RFLP）分析法对初发脑梗死350例，复发脑梗死340例，正常对照组360例患者的MMP-9基因R668Q多态性进行分析。采用ELISA法检测MMP-9的含量，对结果进行比较。结果与正常对照组比较，初发脑梗死组及复发脑梗死组高血压病、吸烟的比率及LDL．C、血清MMP-9水平显著升高（均P〈0．05）。复发脑梗死组LDL—C及血清MMP-9水平显著高于初发脑梗死组（均P〈0．01）。Logistic回归分析显示，血浆MMP-9水平增高是脑梗死初发的独立危险因素（OR=1．336，95％CI：1．031-1．072，P=0．028），但不是脑梗死复发的独立危险因素（OR=0．609，95％CI：0．428-1．006，P〉0．05）。三组MMP-9基因R668Q基因型（X2=0．625，P〉0．05）和等位基因（x2=0．424，P〉0．05）的频率比较，差异无统计学意义。多变量Logistic回归分析显示，MMP-9R668Q基因与脑梗死初发及复发均无明显相关性（OR=0．770，95％CI：0．952-1．541，P〉0．05；OR=0．765，95％CI：0．577-1．376，P〉0．05）。结论MMP-9基因R668Q多态性与动脉粥样硬化性脑梗死初发及复发无明显相关性。Objective To investigate the correlation between matrix metalloproteinase-9 （MMP-9） gene R668Q polymorphism and the atherosclerotie cerebral infarction for the first time onset or recurrence. Methods The MMP- 9 R668Q polymorphism of 350 patients with primary cerebral infarction, 340 patients with a recurrence of atheroselerotic cerebral infarction, and 360 normal controls were measured by PCR-restrietion fragment length polymorphism, and the serum level of MMP-9 were detected by enzyme-linked immunosorbent assay. The resuhs were analyzed. Results Compared with normal control group, the incidence of hypertension and smoking, the serum level of low density lipoprotein cholesterol （LDL-C） and MMP-9 in primary cerebral infarction group and recurrence cerebral infarction group were significantly increased （all P 〈 0. 05 ）. The LDL-C and serum MMP-9 level in recurrence cerebral infarction group were significantly higher than those in primary cerebral infarction group （ all P 〈 0. 01 ）. Multivariate Logistic regression analysis showed that the increased serum MMP-9 level was an independent risk factor for primary cerebral infarction （ OR = 1. 336, 95% CI： 1. 031 - 1. 072, P = 0. 028 ）, but was not an independent risk factor for recurrence cerebral infarction （ OR = 0. 609, 95% CI：O. 428 - 1. 006, P 〉 0. 05 ）. There was no significant difference in the frequencies of genotype （A？ = 0. 625, P 〉 0. 05 ） and allele （X2 = 0. 424, P 〉 0. 05） of MMP-9 gene R668Q among the three groups. Muhivariate Logistic regression analysis showed that R668Q polymorphism had no correlation with cerebral infarction for primary cerebral infarction or recurrence cerebral infarction （ OR = 0. 770, 95% CI： O. 952 - 1. 541, P 〉 0. 05 ; OR = 0. 765, 95% CI： O. 577 - 1. 376, P 〉 0. 05 ）. Conclusion The MMP-9 gene R668Q polymorphism has no correlation with atheroselerotie cerebral infarction for the first time onset or recurrence.

关 键 词：基质金属蛋白酶-9 基因 多态性 动脉粥样硬化性脑梗死 相关性 

分 类 号：R743.3[医药卫生—神经病学与精神病学]