Electroacupuncture alters pain-related behaviors and expression of spinal prostaglandin E_2 in a rat model of neuropathic pain  

作　　者：Jiang Huili Yu Xue Ren Xiujun Tu Ya 

机构地区：[1]School of Acupuncture-moxibustion and Tuina, Beijing University of Chinese Medicine [2]School of Basic Medicine, Beijing University of Chinese Medicine

出　　处：《Journal of Traditional Chinese Medicine》2016年第1期85-91,共7页中医杂志（英文版）

基　　金：Supported by the Grants from the Project of Beijing University of Chinese Medicine:Studies on Effect of Electroacupuncture at Different Acupoint Modules in the Rat Model of Prolapse of Lumbar Intervertebral disc by COX2-PGE2 Pathway and Mechanisms of Acupoints Compatibility(No.JYB22-JS022)

摘　　要：OBJECTIVE： To investigate the role of spinal prostaglandin E2 （PGE2） in electroacupuncture （EA） analgesia and assess the theoretical basis for selection of acupoints in the treatment of neuropathic pain. METHODS： A rat model of neuropathic pain was established. Rats were randomly divided into normal, model, sham, EA 1, EA 2, and EA 3 groups. In EA 1 group, the rats were needled at bilateral L5 Jiaji （EX-B2）, Dachangshu （BL 25）, Weizhong （BL 40） and Kunlun （BL 60）. In EA 2 group, the rats were needled at bilateral Weizhong （BL 40） and Kunlun （BL 60）. In EA 3 group, the rats were needled at bilateral L5 Jiaji （EX-B2） and Dachangshu （BL 25）. EA stimula- tion was administered once daily over 7 days. Motor function and thermal withdrawal latencies were evaluated at 1 day preoperatively and at 3, 5, and 7 days postoperatively. After 7 days of intervention,enzyme-linKed immunosorDnen~ assay （ELISA） Wd3 used to quantify the expression of the spinal PGE2. RESULTS： Rats in the model group exhibited evident hyperalgesia in responses to thermal with- drawal latencies compared with those in the control group （P 〈 0.01）, and EA reversed thermal withdrawal latencies （P 〈 0.01）. The expression level of the spinal PGE2 was significantly higher in the model group than that in the control group and was reversed by EA （P 〈 0.01; P 〈 0.05）. CONCLUSION： The effect of EA on neuropathic pain might alleviate the hyperalgesia state by an inhibition of local prostaglandin E2 secretion.OBJECTIVE: To investigate the role of spinal prostaglandin E_2(PGE_2) in electroacupuncture(EA) analgesia and assess the theoretical basis for selection of acupoints in the treatment of neuropathic pain.METHODS: A rat model of neuropathic pain was established. Rats were randomly divided into normal,model, sham, EA 1, EA 2, and EA 3 groups. In EA 1group, the rats were needled at bilateral L5 Jiaji(EX-B2), Dachangshu(BL 25), Weizhong(BL 40) and Kunlun(BL 60). In EA 2 group, the rats were needled at bilateral Weizhong(BL 40) and Kunlun(BL60). In EA 3 group, the rats were needled at bilateral L5 Jiaji(EX-B2) and Dachangshu(BL 25). EA stimulation was administered once daily over 7 days. Motor function and thermal withdrawal latencies were evaluated at 1 day preoperatively and at 3, 5, and 7 days postoperatively. After 7 days of intervention,enzyme-linked immunosorbnent assay(ELISA) was used to quantify the expression of the spinal PGE_2.RESULTS: Rats in the model group exhibited evident hyperalgesia in responses to thermal withdrawal latencies compared with those in the control group(P < 0.01), and EA reversed thermal withdrawal latencies(P < 0.01). The expression level of the spinal PGE_2 was significantly higher in the model group than that in the control group and was reversed by EA(P < 0.01; P < 0.05).CONCLUSION: The effect of EA on neuropathic pain might alleviate the hyperalgesia state by an inhibition of local prostaglandin E_2 secretion.

关 键 词：Electroacupuncture  Neuralgia DINOPROSTONE Acupuncture analgesia Spinal cord HYPERALGESIA 

分 类 号：R245[医药卫生—针灸推拿学]