阿霉素高产株选育及其发酵工艺优化  

Breeding of High Yield Adriamycin Strain and Optimization Fermentation Process

作　　者：蒋世春 楼惠琴 金洪顺 

机构地区：[1]浙江海正药业股份有限公司,浙江台州318000

出　　处：《激光生物学报》2016年第1期52-56,共5页Acta Laser Biology Sinica

基　　金："十二五"国家重大新药创制项目(2012ZX09201-101-0004)

摘　　要：目的:筛选阿霉素高产菌株,并优化发酵工艺,提高发酵水平。方法:采用微波与氯化锂复合诱变,用正交设计法优化发酵培养基配方,通过摇瓶试验,控制接种量为10%,发酵培养温度29℃,搅拌转速300 r/min,罐压0.04 MPa,空气流量1∶1vvm,保证溶氧浓度40%以上情况下。结果:获得一株阿霉素发酵单位比出发菌株提高83%的高产株;优化后的发酵培养基组成为:淀粉8%,黄豆饼粉3%,Fe SO4·7H2O 0.006%,Ca CO30.3%,其阿霉素发酵效价比原工艺提高2.3倍。结论:采用该方法选育与工艺优化,提高阿霉素发酵单位,有效、快速,结果令人满意,具有工业应用价值。Objective: Process development in fermentation of adriamycin,developing adriamycin fermentation technology to improve the level of fermentation. Methods: Orthogonal design method and combining shake flask test were used to optimizate fermentation medium. The optimum fermentation conditions are as follows: Inoculation quantity 10%,fermentation temperature 29 ℃,stirring rate 300 r / min,tank pressure 0. 04 MPa,air flow 1∶ 1vvm,dissolved oxygen concentration higher than 40%. Results: The fermentation potency of adriamycin was increased by 2. 3 times compared with the original process. Developing fermentation medium was starch 8%,soybean flour 3%,Fe SO4·7H2O 0. 006%,Ca CO30. 3%. Conclusion: This method was effective and rapid with satisfactory results and appling value industry.

关 键 词：阿霉素 高产菌株 选育 发酵 工艺优化 

分 类 号：R978.1[医药卫生—药品]