腺瘤样结肠息肉易感基因（APC）及结直肠癌缺失基因（DCC）基因甲基化在肺癌早期诊断的意义  

Evaluation of the Combination of APC and DCC Gene Methylation in the Early Diagnosis of Lung Cancer

作　　者：马丽颜[1] 梁静[1] 周建龙[1] 

机构地区：[1]广州军区广州总医院,广州510010

出　　处：《现代检验医学杂志》2016年第1期17-20,共4页Journal of Modern Laboratory Medicine

基　　金：国家自然科学基金资助项目（81401752）.

摘　　要：目的探讨腺瘤样结肠息肉易感基因（APC）及结直肠癌缺失基因（DCC）基因甲基化在肺癌早期诊断的意义。方法对245例肺癌患者、150例非恶性肺病患者和40例健康志愿者抽取晨起空腹外周血，应用甲基化特异性PCR法检测APC和DCC启动子区甲基化状态。并对其与临床病理特征等进行相关性分析。结果①肺癌组患者外周血中APC及DCC启动子甲基化阳性率分别为26．53％（65／245）和36．33％（89／245）；非肺癌组患者外周血中APC及DCC启动子甲基化阳性率分别为2．67％（4／150）和8．00％（12／150）；健康志愿者外周血中APC及DCC启动子甲基化阳性率为0；肺癌组与非肺癌组、健康志愿者组相比，APC和DCC基因甲基化检测结果比较，差异均有统计学意义（P〈0．01）。②APC及DCC基因甲基化联合检测，诊断肺癌灵敏度为52．65％，特异度为89．33％，联合检测与单独APC检测相比，敏感度与特异度差异均有统计学意义（P〈0.01）；联合检测与单独DCC检测相比，敏感度有统计学差异（P〈0．01），但是特异度差异不明显（P〉0.05）；③甲基化检测结果与患者性别、年龄、病理类型、病理分化程度、临床TNM分期等没有相关性（P〉0．05）。结论APC和DCC基因甲基化与非小细胞肺癌的发生发展密切相关，可以作为肺癌早期诊断标志物之一。Objective To investigate the significanceof both the adenomatous polyposis coli （APC） and deleted in colorectal carcinoma （DCC） gene methylation in the early diagnosis of lung cancer. Methods 245 patients with lung cancer and 150 patients with non-malignant lung disease patients and 40 healthy volunteers were drawn for the experiment. A methylation specific PCR （MSP） was used to detect the methylation status of APC and DCC in the peripheral blood. Results The positive rates of APC and DCC genes promoter methylation Peripheral blood of patients with lung cancer were 26.53% （65/ 245） and 36.3% （89/245）, respectively. The positive rates of APC and DCC genes promoter methylation Peripheral blood of patients with benign lung diseases were 2.67% （4/150）, 8.00 % （12/150）, respectively. The positive rates of APC and DCC genes promoter methylation Peripheral blood of healthy volunteers were 0. There was a significant difference between patients with the lung cancer, those with the benign lung diseases and Healthy volunteers （P〈0.01）. The sensitivity and specificity of lung cancer was respectively 52.65% and 89. 33% by the combination of APC and DCC genes methylation. Comparing with the separate APC detection, sensitivity and specificity statistical differences were significant in joint measurement （P〈0.01）. Comparing with the separate DCC detection, sensitivity statistical differences were significant, but specificity statistical differences was not obvious （P〉0.05）. There was no correlation between methylation test results and patient gender,age,pathological type,pathological grade,TNM stage and so on （P〉0. 05）. Conclusion APC and DCC gene methylation were closely related to the development of non-small cell lung cancer. They can be used as an early diagnostic marker of lung cancer.

关 键 词：肺癌 腺瘤样结肠息肉易感基因 结直肠癌缺失基因 甲基化 

分 类 号：R735.35[医药卫生—肿瘤] R730.43[医药卫生—临床医学]