异种去细胞角膜基质浸提液对CHL细胞毒性的实验研究  

Research on the Cytotoxicity of the Heterogeneous Acellular Corneal Stromal Leaching Solution on the CHL Cells

作　　者：刘先宁 汪耀[1] 肖湘华[1] 潘士印[1] 银勇 安娜[1] 朱秀萍[1] 

机构地区：[1]陕西省眼科研究所,陕西省眼科学重点实验室,西安市第一医院,西安710002

出　　处：《现代检验医学杂志》2016年第1期112-114,共3页Journal of Modern Laboratory Medicine

基　　金：陕西省科技统筹创新工程计划项目（2012KTCQ03-11）.

摘　　要：目的通过鸵鸟去细胞角膜基质浸提液对中国仓鼠肺纤维细胞（CHL）毒性的实验观察，筛选出浸提液的最大无毒浓度，为进一步完成该去细胞角膜基质材料的染色体畸变实验奠定基础。方法采用细胞培养的方法，将鸵鸟去细胞角膜基质材料制备成浸提液，用浸提原液、1：2，1：4稀释的浸提液与传代培养的CHL细胞共同培养，同时设立阴性、阳性对照，参考MTT比色法评价24h后对细胞生长的影响。结果1：4稀释的浸提液既无毒性，又具有促进细胞生长的作用。结论采用CHL细胞进行细胞毒性试验，依据分级结果并结合细胞形态学特征可筛选出鸵鸟去细胞角膜基质浸提液进行CHL细胞染色体畸变实验的基准无毒浓度。Objective To select the largest non toxic leaching solution concentration through the experimental observation of the cytotoxicity of the ostrich acellular corneal stromal leaching solution to the Chinese hamster lung fibroblasts cells（CHL） for the further chromosome distortion experiment. Methods The leaching solution made from the ostrich acellular corneal stromal material was diluted with concentrate of 1 ：2, 1 ： 4 and the original concentration were used to culture with the CHL cells,the negative and positive control were also set up at the same time,to evaluate the impact on cell growth after 24 hour by MTT colorimetric method. Results The leaching solution diluted with 1 ： 4 was non-toxic,and could promote the growth of the cells. Conclusion Combined with the results of classification and cell morphological features,this eytotoxicity test can be used to screen the best benchmark non-toxic concentrations for the chromosome aberration test of the CHL cells.

关 键 词：鸵鸟去细胞角膜基质浸提液 细胞毒性试验 基准无毒浓度 

分 类 号：R779.65[医药卫生—眼科] R392.4[医药卫生—临床医学]