一株具有石油烃降解性能的交替假单胞菌的筛选和鉴定  

Isolation and Identification of Pseudoalteromonas Strain with Petroleum Degrading Properties

作　　者：郝燕妮[1] 林建国[1] 郭平[1] 塔娜[1] 

机构地区：[1]大连海事大学环境科学与工程学院,大连116026

出　　处：《科学技术与工程》2016年第5期142-146,共5页Science Technology and Engineering

基　　金：国家自然科学基金项目(51208070);中央高校基本科研业务费青年骨干基金项目(3132015079)资助

摘　　要：从大连近岸海域筛选出了1株石油降解菌。通过形态学观察、生理生化反应和16SrDNA基因测序方法鉴定该菌株属于交替假单胞菌属。该菌株接种后,前4 d种群数量增长迅速,为对数增长期,4~11 d为稳定期,11 d后细菌密度下降。在22℃、120 r/min的条件下培养7 d,对柴油降解率可达到41.2%。A petroleum degrading bacterial strain was isolated from oil-contaminated surface seawater of Dalian Port. Based on 16SrDNA sequence analysis, that isolator was identified as Pseudoalteromonas. Cell density in- creased exponentially in the four days incubation and then appeared to plateau phase between days five to ten, after which cell density decreased sharply. Under the condition of 22℃, 120 r/min, the diesel degradation rate was 41.2% after 7 days incubation.

关 键 词：石油污染 石油降解菌 交替假单胞菌 

分 类 号：Q939.9[生物学—微生物学]