小鼠黑素瘤细胞系分泌的外泌体促进间充质基质细胞增殖和迁移  

The exosome secreted by B16 cells promoted the proliferation and migration of mesenchymal stromal cell

作　　者：曹蕾[1] 杨迷芳[2] 平锋锋[3] 田田[5] 王磊 

机构地区：[1]南京医科大学附属无锡第二医院皮肤科,214000 [2]南京医科大学口腔疾病研究所江苏省重点实验室,210029 [3]南京医科大学附属无锡人民医院中心实验室,214000 [4]南京医科大学附属无锡人民医院皮肤科,214000 [5]南京医科大学神经生物学系,210029

出　　处：《中华微生物学和免疫学杂志》2016年第1期42-47,共6页Chinese Journal of Microbiology and Immunology

基　　金：基金项目：南京医科大学重点项目（2015NJMUZD069）;江苏省自然科学基金（BK20130886）

摘　　要：目的研究黑素瘤来源的外泌体（exosome）如何与微环境相互作用。 方法分离并鉴定小鼠黑素瘤细胞培养液上清中的外泌体，并与间充质基质细胞（MSC）共培养。利用免疫荧光观察细胞摄入外泌体情况，CCK-8及transwell实验测定MSC增殖与迁移的变化情况，Western blot检测外泌体对MSC的α平滑肌肌动蛋白（α-SMA）表达的影响。 结果发现MSC在摄取外泌体后，增殖和迁移能力显著上升，α-SMA的表达明显上调。且这些变化都可被TGF-β受体的抑制剂阻断。 结论外泌体可能通过TGF-β诱导MSC构建微环境，促进黑素瘤的生长与转移。ObjectiveTo investigate the interactions between melanoma-derived exosomes and the microenvironment. MethodsThe exosomes were isolated from the culture medium of mouse melanoma cells and then co-cultured with mesenchymal stromal cells （MSC） after identification. Immunofluorescence assay was performed to observe the exosomes engulfed by MSC. CCK-8 and transwell assays were used to evaluate the proliferation and migration of MSC. Effects of the exosomes on the expression of α-smooth muscle actin （α-SMA） in MSC were analyzed by Western blot. ResultsCo-culture of MSC with melanoma cell-derived exosomes enhanced the proliferation and migration of MSC as well as the expression of α-SMA. All of the changes mediated by the exosomes could be blocked by using the inhibitor of TGF-β receptor. ConclusionMelanoma cell-derived exosomes enhanced the proliferation and migration of MSC as well as the expression of α-SMA through TGF-β signaling pathway, which provided an advantageous microenvironment for melanoma progression.

关 键 词：外泌体 黑素瘤 间充质基质细胞 肿瘤微环境 Α平滑肌肌动蛋白 

分 类 号：R739.5[医药卫生—肿瘤]