石榴花中黄酮类成分的提取与含量测定  

Extraction and content determination of flavonoids in pomegranate flower

作　　者：楚红英[1] 罗晓[2] 李瑜 

机构地区：[1]黄河水利职业技术学院环境与化学工程系,河南开封475004 [2]河南中医药大学,河南郑州450003 [3]郑州颐和医院泌尿外科,河南郑州450047

出　　处：《中国酿造》2016年第2期127-130,共4页China Brewing

基　　金：河南省教育厅科学技术研究重点项目(13B150125);河南中医药大学博士启动基金(BSJJ2011-15)

摘　　要：该文对石榴花中黄酮类成分进行提取,并对其含量进行测定,采用正交试验优化石榴花中黄酮提取条件,并用紫外可见分光光度计在波长504 nm处测定其吸光度值并计算其含量。用体积分数70%的乙醇,液料比20∶1(m L∶g),在80℃条件下,黄酮类成分提取效果较好,此时石榴花瓣及花粉中黄酮类成分的含量分别为32.7 mg/g和51.6 mg/g。The flavonoids components were extracted from pomegranate flower and the contents were determined. The optimum extraction conditions of flavonoids from pomegranate flower were optimized by orthogonal experiments and the contents were determined at 504 nm by ultraviolet spectrophotometer. The results showed that the optimal extraction condition were alcohol concentration 70%, liquid-solid ratio 20：1 （ml：g）, temperature 80 ℃, under these conditions, the flavonoid extraction rate from pomegranate flower and pollen were 32.7 mg/g and 51.6 mg/g, respectively.

关 键 词：石榴花 正交试验 黄酮:含量测定 

分 类 号：R284[医药卫生—中药学]