抑郁小鼠模型脑突触体中突触蛋白表达的变化  

Synaptic protein expression of brain synaptosome in depressed mice model

作　　者：吴琼[1] 张小玲[1] 宋靖方 常全忠[3] 

机构地区：[1]漯河医学高等专科学校病理生理学教研室,漯河462000 [2]漯河医学高等专科学校医疗系,漯河462000 [3]漯河医学高等专科学校生理学教研室,漯河462000

出　　处：《解剖学杂志》2016年第1期53-56,共4页Chinese Journal of Anatomy

基　　金：漯河医学高等专科学校博士启动基金

摘　　要：目的：研究抑郁症发病过程中突触蛋白表达的变化。方法：小鼠随机分为对照组和造模组，使用社会失败模型对造模组小鼠造模，之后将其分为对抑郁症敏感组及对抑郁症不敏感组。将以上3组小鼠分别断头取前额叶皮层、海马、纹状体并提取突触小体，通过免疫印迹检测突触小体内突触后致密蛋白95（PSD-95）、桥尾蛋白（gephyrin）及突触蛋白1（synapsin-1）蛋白表达的变化。结果：与对照组相比，在前额叶皮层，对抑郁症敏感组及对抑郁症不敏感组小鼠的PSD-95水平下降，而对抑郁症敏感组小鼠的synapsin-1表达增多；在海马，对抑郁症敏感组及对抑郁症不敏感组小鼠的PSD-95水平下降；在纹状体，对抑郁症不敏感的小鼠gephyrin、synapsin-1蛋白表达显著增加。结论：在抑郁症发病中突触体内兴奋性及抑制性蛋白发生了变化，这些可能与突触功能的紊乱相关。Objective： To study the synaptic protein expression in the process of depression. Methods： Mice were divided into control group and defeat group stochastically. By social defeat model and social avoidance, the defeat group was divided into two groups： susceptible group and unsusceptible group. The mice were sacrificed and the prefrontal cortex, hippocampus, and striatum were dissected to purify a crude synaptoneurosome. Through Western blotting we detected the expression of PSD-95, gephyrin and synapsin-1. Results： Compared with the control group, the PSD-95 level of the prefrontal cortex in susceptible and unsusceptible decreased; synapsin-1 in susceptible mice increased. In the hippocampus, the PSD-95 level of susceptible and unsusceptible mice decreased; in the striatum, the expression of these three proteins showed no significant difference between the control and susceptible group, but gephyrin and synapsin-1 in unsusceptible mice was increased. Conclusion： The expression of synaptosome is changed in depression, which may be related to the synaptic dysfunction.

关 键 词：抑郁症 脑 突触体 突触后致密蛋白95 桥尾蛋白 突触蛋白1 

分 类 号：R749.4[医药卫生—神经病学与精神病学] R-332[医药卫生—临床医学]