NAFLD发病中基因FXR对PPAR-α影响的实验研究  

Experimental study of gene FXR for PPAR-α effects in the pathogenesis of NAFLD

作　　者：邱睿睿 周永健[1] 聂玉强[1] 杜艳蕾[1] 李瑜元[1] 

机构地区：[1]广州医科大学附属市一医院,广东广州510180

出　　处：《胃肠病学和肝病学杂志》2016年第2期159-163,共5页Chinese Journal of Gastroenterology and Hepatology

基　　金：广州市卫生局重大疾病综合防治(2014Y2-000139);广州市医药卫生科技重点项目(2009-ZDi-03)

摘　　要：目的观察法尼基衍生物X受体(FXR)在非酒精性脂肪性肝病(non-alcoholic fatty liver disease,NAFLD)的发病过程中对过氧化物酶体增殖物活化受体-α(PPAR-α)表达的影响。方法将正常小鼠与FXR基因敲除小鼠分成4组,分别喂高脂饲料及普通饲料,检测各组小鼠血清ALT、TC、TG,观察肝脏脂肪变性情况,检测肝脏PPAR-αmRNA及蛋白表达情况。结果模型组血清指标均较实验组显著升高(P<0.05),肝脏HE染色见肝脏内有大量脂肪滴,部分可见炎症细胞聚集,PPAR-αmRNA及蛋白表达水平均明显升高(P<0.05)。结论 FXR基因功能缺陷可能通过上调PPAR-α的表达并改变其在脂质代谢中的功能而引起NAFLD的发生。Objective To investigate whether FXR is correlated with the change of PPAR-et expression in non-alco- holic fatty liver disease （NAFLD）. Methods Normal mice and FXR deficiency mice were divided into four groups （control group, experiment group, model group, observation group） , which were fed with chow diet or high-fat diet for 8 weeks. Then biochemical assays were used to access the serum ALT, TG, TC. Livers FXR expression and PPAR-αexpression were also detected. Results Serum biochemical assays were higher in model group, as well as PPAR-α mRNA and protein expression in livers compared with experiment group （P 〈 0.05）. Moreover, HE staining showed lots of fat- ty droplets and lymphocyte in livers in model group, which indicated liver dysfunction and NASH-iike changes. Conclu- sion During NAFLD development, FXR maybe have a negative correlation with PPAR-α which regulates downstream genes.

关 键 词：非酒精性脂肪性肝病 法尼基衍生物X受体 过氧化物酶体增殖物活化受体-α 

分 类 号：R575[医药卫生—消化系统]