浊点萃取-原子荧光光谱法测定水产品中砷形态  

Determination of arsenic species in seafood samples using cloud point extraction and hydride generation atomic fluorescence spectrometry

作　　者：谭妙瑜 孔冰原 林畅琪 赵旭钦 张泽华[1] 王梅[1] 

机构地区：[1]广东药学院公共卫生学院,广州510310

出　　处：《分析试验室》2016年第2期146-149,共4页Chinese Journal of Analysis Laboratory

基　　金：国家自然科学基金项目(81202251);广东省科技计划项目(2013B060100004;2014A040401085);广州市科技项目(2014J4100085);广东省中医药局项目(20141159)资助

摘　　要：建立了浊点萃取-原子荧光光谱法测定水产品中砷形态的新方法。在pH 4.6,As（III）可与吡咯啶二硫代氨基甲酸铵（APDC）生成疏水性络合物,通过水浴加热,络合物被萃取到Triton X-114表面活性剂相。测定前,表面活性剂相加入0.4 mL Antifoam 204溶液,并用5%（V/V）HCl溶液定容至3 mL。在优化的实验条件下,As（III）在0.2-4.0μg/L范围内与荧光强度有良好的线性关系,方法检出限为0.009μg/L,RSD为3.4%,对于50 mL样品溶液的富集倍数为9.3。方法成功应用于水产品中砷形态分析,加标回收率为95.8%-104.3%。A new method for the determination of trace arsenic species in seafood samples by hydride generation atomic fluorescence spectrometry （HG-AFS） after cloud point extraction was developed. As （III） had formed hydrophobic complex with ammonium pyrrolidinedithiocarbamate （APDC） at pH 4. 6, which was extracted into the non-ionic surfactant-rich phase （Triton X - 114 ） by heating in water bath. Before HG-AFS analysis,Antifoam agent （0. 4 mL） was added to the surfactant-rich phase to quench foaming, followed by dilution to 3 mL with 5% HC1. Under the optimized experimental conditions, the calibration curves for As （III） was linear from 0. 2 to 4. 0μg/L. The detection limit was 0. 009 g/L with RSD of 3.4%. Concentration factor of 9.3 was obtained for a 50 mL sample. The proposed method was successfully used for the determination of arsenic species in seafood samples with recoveries of 95.8% - 104. 3%.

关 键 词：浊点萃取 原子荧光 砷形态 

分 类 号：TS254.7[轻工技术与工程—水产品加工及贮藏工程]