纳米增韧陶瓷的初步生物安全性评价  

Preliminary evaluation of biological safety of new nano-reinforced alumina ceramic

作　　者：沈晴昳 李国强 江国健[2] 

机构地区：[1]上海市口腔病防治院修复科,上海200031 [2]上海应用技术学院材料科学系材料物理教研室,上海201418

出　　处：《口腔医学》2016年第2期97-100,共4页Stomatology

基　　金：国家自然科学基金资助项目(81300917);上海市科学技术委员会科研基金资助项目(12ZR1427100)

摘　　要：目的初步评价牙科全瓷材料纳米增韧(NF)氧化铝陶瓷的生物安全性。方法根据ISO 7405/GB 16886标准,分别通过体外细胞毒性(琼脂覆盖法)、急性溶血、口腔黏膜刺激以及经口急性全身毒性实验,初步评价材料的生物安全性。结果NF陶瓷材料无细胞毒性;溶血率仅0.275%,远低于5%的安全标准;与实验材料接触的颊黏膜未见异常反应,组织切片观察评分为0,无病理性改变;急性全身毒性实验中动物无中毒反应,肝肾等重要组织切片未见病理改变。结论新型纳米增韧氧化铝陶瓷具有较好的生物安全性。Objective To preliminarily evaluate the biological safety of nano-reinforced （NF） alumina ceramic. Methods Accord- ing to ISO 7405/GB 16886 standards, vitro cytotoxicity-test （agar diffusion test）, acute haemolysis-test, oral mucus membrane stimu- lation test and acute systemic toxicity test were carried out to evaluate the biological safety of NF alumina ceramic. Results NF alumi- na ceramic had no cytotoxicity effect in vitro agar diffusion test. The result of acute haemolysis test was 0. 275% , far lower than 5%, which indicated the negative results of heamolysis test. The grade of oral mucous membrane stimulation test in mesocricetus auratus was only 0, which was considered no irritation to mucous membrane according to medical standard. In acute systematic toxicity tests, all of the tested mice survived. By HE staining, no abnormality was observed in kidney and liver tissues. Conclusion NF alumina ceramic is preliminarily proved safe for dental application.

关 键 词：齿科陶瓷 氧化铝陶瓷 生物相容性 体外细胞毒性实验(琼脂覆盖法) 急性溶血实验 口腔黏膜刺激实验 经口急性全身毒性实验 

分 类 号：R783.1[医药卫生—口腔医学]