杧果Mn-MiSOD基因的克隆与表达模式分析  

Molecular Cloning and Expression Analysis of Mn-MiSOD Gene from Mango

作　　者：余海霞[1] 罗聪[1] 徐趁 何新华[1] 

机构地区：[1]广西大学农学院亚热带农业生物资源保护与利用国家重点实验室,南宁530004

出　　处：《植物生理学报》2015年第12期2214-2222,共9页Plant Physiology Journal

基　　金：广西自然科学基金(2013GXNSFDA019011和2014GXNSFBA118102);广西高校科研项目(YB2014009)

摘　　要：本研究在前期研究基础上,采用改良RACE技术和RT-PCR技术克隆了一个杧果Mn-MiSOD基因的全长序列。生物信息学分析显示,该cDNA全长为794 bp,包含一个690 bp的开放阅读框,编码230个氨基酸,分子量为25.67 kDa,等电点为6.3。表达模式分析显示,该基因在各个组织中均表达,但在成熟叶片和果实中表达水平更高。低温胁迫、盐胁迫和PEG干旱胁迫均诱导该基因的表达。热处理、柠檬酸处理、草酸处理、1-MCP处理以及热处理+1-MCP处理上调Mn-MiSOD基因在果实中的表达水平。实验结果表明,Mn-MiSOD基因可能在杧果逆境胁迫和采后生理方面起重要作用。A full-length cDNA sequence of Mn-MiSOD was cloned from mango cultivar ‘SiJi＇ using modifi ed RACE techniques and RT-PCR based on our previous study. Bioinformatics analysis showed that this sequence consists of 794 bp with an open reading frame of 690 bp, encoding a polypeptide of 230 amino acids with a predicted molecular mass of 25.67 kDa and theoretical isoelectric points（pI） of 6.3. Expression analysis indicated that Mn-MiSOD expressed in various tissues, but high expression levels were found in mature leaves and fruits. The expression of Mn-MiSOD could be induced by low temperature, salt and PEG stress. We also found that the post-harvest treatment with heat water, citric acid, oxalic acid, 1-MCP and heat water＋1-MCP could up-regulate Mn-MiSOD gene expression in mango fruit. The experimental results showed that the Mn-MiSOD gene may play important roles in adversity stress and post-harvest physiology in mango.

关 键 词：杧果 Mn-SOD基因 逆境胁迫 采后处理 表达模式 

分 类 号：S667.7[农业科学—果树学]