盐酸去甲基金霉素发酵过程代谢特性与pH的调控的研究  

Study on the Metabolic Characteristics of Demethylvhlortetracyline Sfrand Regulation of pH during Demethylvhlortetracyline Fermentation

作　　者：谢书琴[1,2] 杨淑慎[1] 刘守强 王建国 张增辉 

机构地区：[1]西北农林科技大学,陕西杨陵712100 [2]天方药业有限公司,河南驻马店463000

出　　处：《广东化工》2016年第4期40-41,共2页Guangdong Chemical Industry

基　　金：陕西省社会发展科技攻关项目(2015SF308,野生名贵植物红豆杉产天然抗癌药物紫杉醇的研究)

摘　　要：文章采用上海敖中BMR系列发酵罐发酵生产盐酸去甲基金霉素。通过严格控制氨水和稀料的补入时机和补入量,可以保证去盐酸去甲金霉素具有较高和较稳定的生理活性,并实现菌体由初级代谢向次级代的过渡。在发酵前期pH和湿菌体浓度分别在6.1和40%左右,而中事pH和湿菌体浓度分别在6.0和50%左右时,发酵单位增长速度最快,每24小时去甲金霉素的单位可增加1000 ug/m L左右。The paper adopts BMR series fermentation production of hydrochloric acid in Shanghai Ao demethylchlortetracycline. Through strict control of ammonia and thinner feeding time and feeding amount, can guarantee to demeclocycline hydrochloride has a higher and more stable physiological activity, and to achieve the transition to secondary metabolism by primary cell generation. In the early stage of fermentation and pH wet cell concentrations were about 6.1 and 40 %, while the pH and wet biomass concentration respectively in 6 and 50 %, was the fastest growth, every 24 hours demethylchlortetracycline units can be increased about 1000 ug/m L.

关 键 词：去甲金霉素 发酵 PH 补料 

分 类 号：TQ465.4[化学工程—制药化工]