萘降解菌的分离鉴定及在污染土壤生物修复中的应用  

Isolation and Identification of Naphthalene Degrading Bacterium and Its Role in Bioremediation of Naphthalene-contaminated Soil

作　　者：廉景燕[1] 吕勃熠 刘金彪[1] 史小凤[1] 黄磊[1] 

机构地区：[1]天津理工大学化学化工学院,天津300384

出　　处：《南开大学学报（自然科学版）》2015年第6期92-98,共7页Acta Scientiarum Naturalium Universitatis Nankaiensis

基　　金：天津市应用基础及前沿技术研究计划资助项目(15JCQNJC08800)

摘　　要：从大庆油田的土壤中筛选出了一株高效萘降解菌株DQ-1,初步鉴定为假单胞菌属(Pseudomonas sp.).该菌接种到以3 g/L萘为唯一碳源的无机盐培养基中,30℃培养4 d,其降解率达到98.6%.研究了Cu^(^(2+))、Cr^(6+)、Fe^(3+)、Zn^(2+)和Pb^(2+)对菌株DQ-1生长的抑制情况,生长抑制效应由强到弱的顺序为Zn^(2+)>Cr^(6+)>Cu^(2+)>Fe^(3+)>Pb^(2+),并利用直读电感耦合等离子体发射光谱仪(ICP-OES,美国VARIAN公司)测定培养体系中重金属离子的含量,结果表明重金属离子最终吸附在菌株细胞壁上.将DQ-1菌株接种到含有2.5 mg/g萘的灭菌土壤中,经过11d室温培养之后,萘的去除率为97.9%.A naphthalene-degrading strain DQ-1 was isolated from oil contaminated soil of Daqing Oil Field and identified as Pseudomonas sp., which could utilizing naphthalene as a sole carbon source for growth and degrading naphthalene up to 98.6% of existed in minimal medium （3 g/L） within 4 days. Study on the inhibition of Cu^2＋, Cr^6＋, Fe^3＋, Zn^2＋ and Pb^2＋ on the growth of strain DQ-1, the inhibition effect follows the order of Zn^2＋〉 Cr^6＋〉 Cu^2＋〉 Fe^3＋ 〉 Pb^2＋. With the test of ICP-OES, it proved that the bio- sorption as a possible mechanism of heavy metal resistance in DQ-1. After inoculating strain DQ-1 into the autoclaved soils containing 0.25% naphthalene and incubating it at room temperature for 11 days, 97.9% naphthalene was removed.

关 键 词：萘 生物降解 假单胞菌 重金属 土壤修复 

分 类 号：X172[环境科学与工程—环境科学]