母猪杀婴行为的全基因组关联分析  

Genome-Wide Association Study on Maternal Infanticide Behavior in Pigs

作　　者：杨慧 方绍明 黄晓畅 陈从英 张志燕 

机构地区：[1]省部共建猪遗传改良与养殖技术国家重点实验室,南昌330045

出　　处：《畜牧兽医学报》2016年第2期241-248,共8页ACTA VETERINARIA ET ZOOTECHNICA SINICA

基　　金：国家自然科学基金(30760164);江西省自然科学基金(2008GQN0031)

摘　　要：本研究测定了288头来自白色杜洛克×二花脸F2群体的母猪杀婴行为表型,旨在定位出影响母猪杀婴行为的QTL。基于整个家系及已经扫描个体的60KSNP芯片信息推测出本试验288头个体60K的基因型。随后,我们利用R语言GenABEL软件包的广义混合线性模型对母猪杀婴行为表型进行全基因组关联分析,定位影响母猪杀婴行为的显著关联SNP位点。结果,在SSC2和SSC8上共发现50个与母猪杀婴显著关联的SNPs位点,其中有21个SNPs位于SSC8：35.05-47.54 Mb区域达到基因组显著水平,在SSC2上只有1个SNP位点达到染色体显著水平。通过搜寻显著相关SNP所在区域内的注释基因,发现了12个可能影响母猪杀婴行为的重要位置候选基因。该研究为进一步鉴别影响母猪杀婴行为的主效基因和因果突变位点提供了研究基础。Maternal infanticide behavior in 288 sows from a White Duroc× Erhualian F2 resource population was measured.The aim of the study was to identify the QTL affecting maternal infanticide behavior.According to pedigree information and genotyped samples with 60 Kchip,we speculated the 60 Kgenotypes of the 288 tested individuals.Furthermore,agenome-wide association study was carried out to identify the significant genomic loci associated with maternal infanticide behavior using GenABEL packages in R software.We detected a total of 50 significant SNPs associated with maternal infanticide behavior on SSC2 and SSC8.Totally 21 SNPs on SSC8：35.05-47.54 Mb reached the genome-wide significant level.Only 1SNP on SSC2：89.5 Mb reached the suggestive significance.The candidate genes around significant SNPs were searched based on its functional annotations.A total of 12 genes were found to be the most important candidate genes for maternal infanticide behavior.This study provided a foundation for identification of major gene or causative mutation influencing the maternal infanticide behavior.

关 键 词：猪 全基因组关联分析 母猪杀婴 候选基因 

分 类 号：S828[农业科学—畜牧学] S813.3[农业科学—畜牧兽医]