虾青素抑制MCD饮食诱导的小鼠非酒精性脂肪肝纤维化  

Astaxanthin inhibits fibrosis in mice with non-alcoholic fatty liver disease induced by MCD diet

作　　者：邹堂斌[1] 黄明元[1] 冼叶明 修良昌[1] 

机构地区：[1]广东医学院公共卫生学院,广东东莞523808 [2]东莞市大岭山医院,广东东莞523820

出　　处：《现代预防医学》2016年第4期700-703,共4页Modern Preventive Medicine

基　　金：国家自然科学基金(81302416);广东省科技计划项目(2013B031800016;2014A020212297);广东省高校优秀青年教师培养计划项目(YQ201405);东莞市科技计划项目(2014108101053)

摘　　要：目的通过蛋氨酸-胆碱缺乏(Methionine-Choline-Deficient,MCD)饮食诱导的小鼠脂肪性肝炎(NonAlcoholic Steatohepatitis,NASH)模型研究虾青素抗非酒精性脂肪肝纤维化效应及其机制。方法将30只雄性C57BL/6J小鼠随机分为对照组、模型组和虾青素组,每组10只。模型组饲喂MCD饲料,对照组在MCD饲料基础上每kg饲料添加3 g L-蛋氨酸和2 g胆碱;虾青素组在MCD饲料基础上用虾青素口服液灌胃(20 mg/kg.bw),每周2次。4周后,称量各组小鼠体重、肝重并计算肝脏指数;测定小鼠血清TG、TC、ALT、AST及肝脏TG、TC水平。肝组织苏木素和伊红(Hematoxylin and Eosin,HE)染色观察组织形态学变化;荧光定量PCR检测α-平滑肌肌动蛋白(α-Smooth Muscle Actin,α-SMA)和Ⅰ型胶原的m RNA表达水平。结果模型组小鼠血清ALT、AST水平,肝脏TG、TC水平及α-SMA和Ⅰ型胶原的m RNA表达与对照组相比显著升高(P<0.05),上述指标虾青素组与模型组相比显著降低(P<0.05);模型组和虾青素组小鼠血清TG、TC水平与对照组相比显著降低(P<0.05)。HE染色结果表明,模型组肝组织可见大量脂肪变性,而虾青素组脂肪变性程度明显减轻。结论虾青素可以改善肝功能,且可能通过调控α-SMA和Ⅰ型胶原基因的表达减缓MCD饮食诱导的非酒精性脂肪肝纤维化进程。Objective The study aimed to investigate the anti-fibrogenic effect of astaxanthin on non-alcoholic fatty liver disease （NAFLD） induced by methionine-choline-deficient （MCD） diet and its mechanism. Methods Thirty male C57BL/6J mice were randomly divided into a control group, a model group, and a astaxanthin group. Model group mice were fed with MCD diet; control group mice were fed with MCD diet but supplemented with L-methionine （3g/kg） and chloride （2g/kg）; astaxanthin group mice were gavaged with astaxanthin oral liquid （20 mg/kg, bw） on the basis of MCD diet, 2 times a week. After four weeks, the body weight and liver weight of mice were recorded and the index of liver was calculated. The mice were sacrificed to take serum samples for biochemical measurements and liver samples for HE staining and RT-PCR. Results Serum ALT, AST level, hepatic TG, TC level and mRNA expression of α-SMA, collagen I in model group mice significantly higher than those in control group （P〈0.05）, the above index were significantly degraded in astaxanthin group compared with model group （P〈0.05）. Serum TG, TC level in model and astaxanthin group mice significantly lower than control group （P〈0.05）. The results of HE staining showed that a lot of steatosis in hepatic tissue of model group. However, the degree of steatosis was alleviated in astaxanthin group. Conclusion Astaxanthin can improve liver function and inhibit fibrosis possibly by downregulating the mRNA expression of α -SMA and collagen I with NAFLD induced by a MCD diet.

关 键 词：虾青素 蛋氨酸-胆碱缺乏饮食 非酒精性脂肪性肝病 肝纤维化 

分 类 号：R319[医药卫生—基础医学]