PEDF对肺腺癌A549细胞迁移、侵袭能力和转移相关蛋白表达水平的影响  

Effects of PEDF on the migration and invasion of lung adenocarcinoma A549 cells and the expression of metastatic-related proteins

作　　者：程宇[1] 束军[1] 徐胜[1] 李晓峰[1] 

机构地区：[1]安徽医科大学第四附属医院呼吸内科,合肥230022

出　　处：《安徽医科大学学报》2016年第3期345-350,共6页Acta Universitatis Medicinalis Anhui

基　　金：安徽省自然科学基金面上项目(编号:1308085MH141);安徽医科大学科研基金资助项目(编号:2010xkj115)

摘　　要：目的观察色素上皮衍生因子(PEDF)对肺腺癌A549细胞迁移、侵袭能力以及对转移相关蛋白如纤维连接蛋白(FN)和基质金属蛋白酶9(MMP-9)表达水平的影响。方法设不加PEDF的肺腺癌A549细胞为阴性对照组,PEDF干预组细胞分别加入180、360和720 nmol/L PEDF;Transwell检测PEDF对A549细胞迁移和侵袭能力的影响;半定量RT-PCR法检测PEDF对FN、MMP-9 mRNA表达水平的影响;ELISA法检测PEDF对A549细胞培养上清液中和细胞内的FN、MMP-9蛋白表达水平的影响。结果 PEDF干预组的A549细胞迁移细胞数和侵袭细胞数均显著低于阴性对照组(P<0.05),随着PEDF浓度的增加,迁移和侵袭细胞数逐渐减少(P<0.05)。各浓度PEDF干预组A549细胞的FN、MMP-9表达水平较阴性对照组显著降低(P<0.05),随着PEDF浓度的增加,FN、MMP-9表达水平逐渐降低(P<0.05)。结论 PEDF在体外能显著抑制肺腺癌A549细胞的迁移和侵袭能力,能显著抑制转移相关蛋白(FN、MMP-9)的表达水平。Objective To observe the effects of pigment epithelium-derived factor （PEDF） on the migration and invasion of human lung adenocarcinoma A549 cell, as well as the expression of metastatic-related proteins including fibronectin（FN） and matrix metalloproteinase 9 （MMP-9）. Methods A549 cells cultured without PEDF were defined as negative control group, the cells in PEDF intervention group were added 180, 360 and 720 nmol/L PEDF, respectively. The effects of PEDF on the migration and invasion of A549 cell were detected by Transwell assay. The effects of PEDF on the expression of FN and MMP-9 mRNA were detected by semi-quantitative RT-PCR assay, meanwhile the protein of them in supernatant of cell culture and within cells was detected by ELISA. Results The numbers of migrating ceils and invasive cells in PEDF intervention group were significantly lower than those in the negative control group （ P 〈 O. 05 ）. With the increase of PEDF concentration, the numbers of migrating and invasve cells decreased by degrees （P 〈 0.05 ）. The expression of FN and MMP-9 in A549 cells was significantly lower than that in the control group （P 〈 0. 05 ） and the expression level was remarkably decreased by degrees with the increase of PEDF concentration （P 〈 0. 05 ）. Conclusion PEDF can significantly inhibit the migration and invasion of A549 cells in vitro, furthermore, PEDF can significantly inhibit the expression of metastasis associated proteins （ FN and MMP-9）.

关 键 词：色素上皮衍生因子 肺腺癌 转移 迁移 侵袭 

分 类 号：R734.2[医药卫生—肿瘤] R73-37[医药卫生—临床医学]