HLA-G慢病毒表达载体的构建及病毒颗粒的包装  

Construction of lentiviral vector carrying HLA-G gene and packing of the lentiviruses

作　　者：廖贵益[1] 钟金彪 赵飞[1] 朱道方[1] 

机构地区：[1]安徽医科大学第一附属医院泌尿外科,合肥230022

出　　处：《安徽医科大学学报》2016年第3期457-460,共4页Acta Universitatis Medicinalis Anhui

基　　金：安徽高校省级自然科学研究项目(编号:KJ2014A123)

摘　　要：设计合成全长人类白细胞抗原G（HLA-G）基因系列,在克隆质粒pLV-EF1a-EGFP-N的基础上构建HLA-G基因慢病毒表达载体,测序鉴定pLV-EF1a-EGFP-N-HLA-G构建成功。与包装质粒（pGag/Pol、pRev、pVSV-G）转染293T包装细胞,包装出高效感染率的慢病毒颗粒,测定病毒滴度为1×109TU/ml。HLA-G慢病毒表达载体的构建及病毒颗粒的包装为转染HLA-G诱导移植肾免疫耐受的研究奠定了基础。The human leucocyte antigen-G （HLA-G）gene was synthesized. According to gene sequencing, the lentiviral vector carrying HLA-G was constructed successfully on the basis of cloning plasmid pLV-EF1 a-EGFP-N. The constructed lentiviral vector was transferred into 293T package cell with pGag/Pol, prey and pVSV-G and the lentiviruses with high infection efficiency were produced. The virus titer was 1 x 109 TU/ml. Construction of lentiviral vector carrying HLA-G gene and packing of the lentiviruses laid the material foundations for the study of HLA-G gene in inducing immune tolerance of transplanted kidney.

关 键 词：HLA-G 慢病毒 基因工程 免疫耐受 

分 类 号：R392.4[医药卫生—免疫学]