多种沙门氏菌分离培养基效果比较及在实验动物和垫料检测中的应用  

Comparison of Several Separation Media Effect for Detection of Salmonella and Application in Laboratory Animals and Bedding

作　　者：邢进[1] 冯育芳[1] 岳秉飞[1] 贺争鸣[1] 

机构地区：[1]中国食品药品检定研究院实验动物资源研究所,北京100050

出　　处：《实验动物科学》2015年第6期13-17,21,共6页Laboratory Animal Science

基　　金：国家科技支撑计划项目:实验动物新品种的种群建立与质量标准化研究(No.2011BAI15B01)

摘　　要：目的改进实验动物沙门氏菌分离培养方法,建立垫料中沙门氏菌检测方法。方法参考国内外沙门氏菌检测方法,用不同分离培养基对沙门氏菌、大肠杆菌和变形杆菌进行培养。选用最佳筛选效果培养基对动物肠道和垫料样品进行检测。结果 BPW预增菌、MKTTn选择性增菌转种XLT4培养基分离沙门氏菌的效果优于其他培养基,在1015份动物样品中检测出3份沙门氏菌阳性,能够检测出垫料中1CFU/g的沙门氏菌。结论改进的沙门氏菌分离培养方法检出率高于现有国标方法,结果准确,可用于实验动物沙门氏菌及垫料的检测。Objective To improve isolate culture method of Salmonella in laboratory animal, and develop detection method of Salmonella for animal bedding. Method According to variety of domestic and international Salmonella detection methods, 10 strains of Salmonella, Escherichia coli and Proteus were cultured by these methods. The best media were selected to detect animal intestines and bedding samples. Result BPW, MKTTn and selective enrichment medium XLT4 were better than other media for isolating Salmonella, its detection limits was 1CFU/g for Salmonella in bedding. In addition, three samples were positive in the 1015 animal samples by this method. Conclusion The improved method of isolation and culture of Salmonella detection was superior to the GB method, and accurate. Therefore, it can be used for detecting salmonella in laboratory animal and bedding.

关 键 词：沙门氏菌 实验动物 检测方法 分离培养 垫料 

分 类 号：R378[医药卫生—病原生物学]