P2X3受体对大鼠海马CA1区锥体神经元兴奋性的影响  

Effects of P2X3 receptor on neuronal excitability in hippocampal CA1 pyramidal cells of rats

作　　者：周鑫[1] 陈阳美[1] 

机构地区：[1]重庆医科大学附属第二医院神经内科,重庆400010

出　　处：《重庆医科大学学报》2016年第1期14-17,共4页Journal of Chongqing Medical University

基　　金：国家自然科学基金资助项目(编号:81171225)

摘　　要：目的:研究P2X3受体(P2X3 receptor,P2X3R)在海马CA1区的定位及对CA1区锥体细胞兴奋性的影响。方法:免疫荧光技术对P2X3R在海马CA1区表达进行定位,SD鼠麻醉后断头取脑,修块后应用振动切片机切出350μm脑片并用正常人工脑脊液26℃恒温孵育1 h,通过膜片钳全细胞记录技术,给予P2X3R选择性拮抗剂AF-353和非选择性激动剂α,β-Me ATP干预,记录锥体细胞动作电位发放频率的改变。结果:P2X3R在海马区主要表达于神经元上,在星形胶质细胞不表达。P2X3R拮抗剂AF-353减慢锥体细胞动作电位发放频率减小其兴奋性[(0.788±0.163)Hz vs.(0.352±0.079)Hz,t=7.32,P=0.002],α,β-Me ATP则加快动作电位发放频率[(0.715±0.186)Hz vs.(1.610±0.454)Hz,t=-4.97,P=0.008],增加兴奋性。结论 :P2X3R在海马区主要表达于神经元树突及胞质并且影响神经元兴奋性,可能参与了兴奋性增高有关疾病的发生发展过程。Objective：To investigate the effects of P2X3 receptor（P2X3R）on neuronal excitability in hippocampal CA1 pyramidal cells of rats. Methods：The localization of P2X3 R was detected by immunofluorescence. Sprague-Dawle rats were euthanized via decapitation after anesthetization and the brain tissues were trimmed and placed in a vibratome to obtain a slice thickness of 350 μm.The slices then were incubated in normal artificial cerebrospinal fluid at 26 ℃ for 1 h. Intervention of AF-353 and α,β-Me ATP was performed and the action potentials（APs）frequency was recorded via whole cell patch-clamp technique. Results：The P2X3 R was located at the neuron instead of the astrocyte in hippocampal CA1 region. The antagonist of AF-353 decelerated the APs frequency and reduced cellular excitability[（0.788±0.163）Hz vs.（0.352±0.079）Hz,t=7.32,P=0.002] whereas α,β-Me ATP agonist had the reverse effects[（0.715±0.186）Hz vs.（1.610±0.454）Hz,t=-4.97,P=0.008]. Conclusion：P2X3R effect neuronal excitability and may participate in the process and development of diseases related to hyperexcitability.

关 键 词：P2X3受体 兴奋性 癫痫 阿尔茨海默病 

分 类 号：R741.05[医药卫生—神经病学与精神病学]