头部浅低温联合PTEN活性抑制预处理对大鼠全脑缺血-再灌注损伤的影响  

Effects of combination of mild head hypothermia and inhibition of PTEN activity preconditioning on global cerebral ischemia- reperfusion injury in rats

作　　者：杨树忠[1] 张志强[1] 张山[1] 李艳丽[1] 徐雪[1] 蔡劲松[1] 

机构地区：[1]河北医科大学第二医院麻醉科,河北石家庄050000

出　　处：《中国急救医学》2016年第2期159-162,I0002,共5页Chinese Journal of Critical Care Medicine

基　　金：河北省自然科学基金（H2012206012）

摘　　要：目的探讨头部浅低温联合磷酸酶和张力蛋白同源等位基因PTEN活性抑制预处理对大鼠全脑缺血一再灌注损伤的影响。方法健康雄性Wistar大鼠60只，体质量250～280g，采用随机数字表法将其分为五组（n=12）：假手术组（S组）、全脑缺血-再灌注组（IR组）、BPV（pic）抑制PTEN活性预处理组（B组）、浅低温预处理组（MH组）和PTEN活性抑制联合头部浅低温预处理组（MB组）。采用四血管阻断法建立大鼠全脑缺血-再灌注模型，缺血15min后行再灌注8h。每组随机取6只大鼠，采用免疫组织化学法测定大鼠海马CAl区P—PTEN、Bax和Bcl-2蛋白的表达水平。每组取另6只大鼠，用于测定海马组织中丙二醛（MDA）含量和SOD的活性。结果与s组相比，IR组P—PTEN蛋白表达减少，Bax蛋白表达增多，Bcl-2／Bax降低，MDA含量增多，超氧化物歧化酶（SOD）活性降低；与IR组相比，B组、MH组和MB组P—PTEN和Bcl-2蛋白表达增多，Bax蛋白表达减少，Bcl-2／Bax升高，MDA含量减少，B组SOD活性不变，MH组和MB组SOD活性增高；与B组或MH组相比，MB组P-PTEN和Bcl-2蛋白表达增多，Bax蛋白表达减少；Bcl-2／Bax升高，MDA含量减少，SOD活性增高（P〈0．05）。结论头部浅低温预处理、PTEN活性抑制预处理及两者联合均可减轻大鼠全脑缺血一再灌注损伤，且联合应用比单独应用的神经保护作用增强，其机制可能与神经元凋亡的抑制和活性氧生成减少有关。Objective To investigate the effects of mild head hypothermia and combination of inhibition of PTEN activity preconditioning on global cerebral ischemia - reperfusion injury in rats. Methods Sixty healthy male Wistar rats weighing 250 - 280 g were randomly divided into five groups （n = 12 each） ： group I sham operation （S） ; group H global cerebral ischemia -reperfusion injury （IR） ; group m inhibition of PTEN activity preconditioning （B） ; group Ⅳ mild hypothermia preconditioning （MH） and group V combination of inhibition of PTEN activity and mild hypothermia preconditioning （MB）. Global cerebral ischemia- reperfusion was produced by modified Pulsinelli＇s four- vessel occlusion for 15 min followed by 8 h reperfusion in groups Ⅱ-Ⅴ. Global cerebral ischemia was confirmed by isoelectric EEG, dilated pupil and pale conjunctiva. In group Ⅲ and V BPV （pic） was given intraperitoneally before global cerebral ischemia while in group H and IV rats were given an equalvolume nomal saline. In group Ⅳ and V nasopharyngeal cooling was applied to reduce head temperature before global cerebral ischemia. In group I alar foramina and common carotid arteries were exposed but no cerebral ischemia was performed. At the end of 8 h reperfusion, six rats were killed in each group for determination of expression of P - PTEN, Bax and Bcl - 2, MDA content and SOD activity. Results Inhibition of PTEN activity, mild hypothermia and their combination increased the expression of P - PTEN and Bcl - 2/Bax ratio and decreased MDA content. Expression of P - PTEN and Bcl -2/Bax ratio were significantly higher while MDA content was significantly lower and SOD activity was significantly higher in group MB than that in group B or group MH. Conclusion Inhibition of PTEN activity preconditioning, mild hypothermia preconditioning and their combination all can alleviate global ischemia - reperfusion injury in rats and all have neuroprotective effect. Combination of inhibition of PTEN activity and mild head hypothe

关 键 词：基因 磷酸酶和张力蛋白同源等位基因(PTEN) 低温 缺血一再灌注损伤 脑 

分 类 号：R743.3[医药卫生—神经病学与精神病学]