丁苯酞注射液对蛛网膜下腔出血大鼠早期脑损伤的影响  

THE RESEARCH OF BUTYLPHTHALIDE INJECTION AFFECT ON EARLY BRAIN INJURY OF RAT MODEL OF SUBARACHNOID HEMORRHAGE

作　　者：李珺[1] 张琪 钱东翔[1] 郭炜[1] 王智坚[1] 

机构地区：[1]广州医科大学附属第三医院神经外科,广州510080 [2]广州市增城人民医院神经外科,广州511300

出　　处：《广西医科大学学报》2015年第6期898-901,共4页Journal of Guangxi Medical University

基　　金：广州医科大学附属第三医院青年科研基金资助项目(No.2012Y04)

摘　　要：目的:观察丁苯酞(NBP)注射液对蛛网膜下腔出血(SAH)大鼠早期脑损伤(EBI)的影响,探讨其效果及可能机制。方法:建立SAH大鼠模型,将60只大鼠随机分为6组,分别为空白对照组(非手术组)、生理盐水对照组(NS对照组)、NBP组,每组又根据时点不同分为12h组与24h组,检测各组间不同时点丙二醛(MDA)、肿瘤坏死因子-α(TNF-α)、白介素-1β(IL-1β)、细胞间黏附分子-1(ICAM-1)以及细胞凋亡情况。结果 :NS对照组和NBP组的MDA、TNF-α、IL-1β、ICAM-1及细胞凋亡率均较非手术组升高(P<0.05),同一时点内(12h或者24h),NBP组MDA、TNF-α、IL-1β、ICAM-1及细胞凋亡率较NS对照组降低(P<0.05)。结论:在SAH后早期,MDA、TNF-α、IL-1β、ICAM-1的水平以及细胞凋亡率会升高,NBP在一定程度上能够减少这些炎性分子的表达,减轻炎性反应的发生,并降低SAH后细胞凋亡率,对SAH后的EBI起到一定的保护作用。Objective： To observe the affect of butylphthalide injection on early brain injury after subarach noid hemorrhage in rats, and explore its effects and possible mechanisms. Methods： Creating rat model of SAH,60 rats were divided into six groups randomly：blank control group, NS group, NBP group, and each group was divided into 12 hours group and 24 hours group. MDA,TNF-α,IL-β,ICAM-1 and condition of cell apoptosis were detected in each group in 12 hours and 24 hours. Results： The level of MDA,TNF -α,IL -β,ICAM-1 and condition of cell apoptosis all increased in NS group and NBP group than those in blank control group（ P 〈0.05） ,in the same time（I2 hours or 24 hours） ,the level of MDA,TNF-α,IL-1β,ICAM- 1 and condition of cell apoptosis all decreased in NBP group than those in NS group. Conclusion： The level of MDA,TNF-α,IL-1β,ICAM-1 and cell apoptosis increase in the early stage of SAH,NBP can improve the condition of the early brain injury after the SAH through reducing the expression of MDA,TNF-α, IL-lβ, ICAM-1,easing the Inflammation and lowering the cell apoptosis to some extent.

关 键 词：丁苯酞 蛛网膜下腔出血 早期脑损伤 丙二醛 肿瘤坏死因子-α 白介素-1Β 细胞间黏附分子-1 

分 类 号：R-33[医药卫生]