基于AuNPs/rGO复合材料的电化学生物传感器用于Cu^(2+)痕量检测的研究  

Research on Cu^(2+) trace detection of electrochemical biosensor based on composites of gold nanoparticles/reduced graphene oxide

作　　者：梁崇佳 郭川磐 冯孝中[1] 王明花[1,3] 张治红[1,3] 

机构地区：[1]郑州轻工业学院材料与化学工程学院,河南郑州450001 [2]河南省体育运动学校,河南郑州450045 [3]郑州轻工业学院环境污染治理与生态修复河南省协同创新中心,河南郑州450001

出　　处：《轻工学报》2016年第1期96-104,共9页Journal of Light Industry

基　　金：国家自然科学基金项目(51173172);河南省科技开放合作项目(132106000076);河南省高等学校重点科研项目(15A610024)

摘　　要：采用液相化学共还原法制备AuNPs/rGO复合材料,再利用XRD和SEM对其晶型和表面形貌进行考察,通过XPS对其固定DNA前后的化学组成进行分析,采用电化学交流阻抗法对其用于Cu2+痕量检测过程中电化学性能变化进行测试.结果表明,所制备的AuNPs/rGO复合材料结晶性良好,与氧化石墨烯(GO)的表面形貌相比,AuNPs/rGO复合材料表面呈现出褶皱现象,并且表面包裹了许多金纳米颗粒.XPS分析表明DNA可成功地固定在AuNPs/rGO复合材料表面.基于AuNPs/rGO复合材料的电化学生物传感器对Cu2+的最低检测限为0.02 nmol/L,比其他检测手段更加灵敏,且具有优良的选择性和重复性.The composites of gold nanopartieles/reduced graphene oxide （AuNPs/rGO） was synthesized by liquid phase one-step co-reduction method. The crystal structure and the surface morphology were characterized by XRD and SEM. XPS was employed to characterize the chemical components before and after DNA absorbed on AuNPs/rGO composites. Additionally, electrochemical impedance spectroscopy （EIS） was used to measure the electrochemical property changes of Cu^2 ＋ detection. The results showed that the AuNPs/rGO composites had good crystal structure. Comparing with graphene oxide, there were wrinkles and gold nanoparticles on the AuNPs/rGO composites surface. The XPS spectra showed that DNA was immobilized on the AuNPs/ rGO composites surface successfully. And the developed electrochemical biosensor exhibited high sensitivity toward the determination Cu^2 ＋ with a low detection limit of 0.02 nmol/L. This biosensor aslo showed high selectivity and reusability.

关 键 词：AuNPs/rGO复合材料 电化学生物传感器 Cu^2+痕量检测 

分 类 号：TS07[轻工技术与工程]