转锰过氧化物酶基因酵母的建立及其降解秸秆木质素能力的研究  

Study on establishment of recombinant Pichia pastoris with manganese peroxidase gene and its ability of degrading lignin in corn straw

作　　者：王娇[1,2] 王秋文[1] 尹清强[1] 岳道友[2] 常娟[1] 宋安东[3] 党晓伟 刘洪兵 

机构地区：[1]河南农业大学牧医工程学院,河南郑州450002 [2]濮阳职业技术学院,河南濮阳457000 [3]河南农业大学生命科学学院,河南郑州450002 [4]河南德邻生物制品有限公司,河南新乡466000 [5]泌阳县畜牧局,河南泌阳463700

出　　处：《河南农业大学学报》2015年第6期817-821,共5页Journal of Henan Agricultural University

基　　金：郑州市科技创新团队(112PCXTD339);河南省高校科技创新团队支持计划(15IRTSTHN014)

摘　　要：为了提高锰过氧化物酶基因的表达产量,从黄孢原毛平革菌中获取锰过氧化物酶基因,并将其转化至毕赤酵母中。在液态发酵培养条件下,重组酵母与原始黄孢原毛平革菌所分泌的锰过氧化物酶活性分别为0.317 8U·m L^(-1)和0.197 2 U·m L^(-1)(P<0.05);重组酵母所表达酶的最适温度和p H值分别为40℃和4.5,与原始酶的生化特性基本一致。在含有玉米秸秆的液体培养基中,重组酵母对秸秆中木质素降解率达到24.09%,而黄孢原毛平革菌对木质素的降解率为16.53%(P<0.05)。In order to enhance manganese peroxidase production,the manganese peroxidase gene was isolated from Phanerochaete chrysosporium and transformed into Pichia pastoris（ X-33）. Under the same conditions of liquid-state incubation for the recombinant Pichia pastoris and Phanerochaete chrysosporium,manganese peroxidase activity was 0. 317 8 U·m L- 1and 0. 197 2 U·m L- 1,respectively（ P 0. 05）. The optimal temperature and p H value of the expressed enzyme were 40 ℃ and 4. 5,which was the same as the native enzyme. The liquid-state fermentation with corn straw showed that lignin degradation rates in corn straw were 24. 09% and 16. 53% for the recombinant Pichia pastoris and Phanerochaete chrysosporium,respectively（ P 0. 05）.

关 键 词：锰过氧化物酶 基因表达 毕赤酵母 木质素降解 

分 类 号：S816[农业科学—饲料科学]