白藜芦醇对食管鳞癌Eca-109细胞侵袭迁移及EMT的影响  

Effect of resveratrol on the invasion migration of Eca-109 cells and epithelial-mesenchymal transition in esophageal squamous cell carcinoma

作　　者：臧春宝[1] 刘云琴[1] 葛宁[1] 冯继红[2] 赵芳芳[3] 

机构地区：[1]安徽省肿瘤医院放疗科,安徽合肥230031 [2]遵义医学院附属医院肿瘤科,贵州遵义563003 [3]安徽省肿瘤医院表观遗传学实验室,安徽合肥230031

出　　处：《蚌埠医学院学报》2015年第12期1617-1620,1623,共5页Journal of Bengbu Medical College

基　　金：国家自然科学基金项目(81560407);安徽省自然科学基金(1608085QH214)

摘　　要：目的：研究白藜芦醇（RES）对食管鳞癌Eca-109细胞侵袭迁移及上皮间质转化（EMT）的影响。方法：用不同浓度（50和100μmol/L）的RES处理Eca-109细胞,采用细胞划痕实验、Transwell小室实验检测细胞迁移侵袭能力,荧光定量PCR检测EMT蛋白E-cadherin、N-cadherin、Vimentin和MMP-2、MMP-9 mRNA的表达,Western blot检测E-cadherin、N-cadherin、Vimentin、MMP-2和MMP-9蛋白表达。结果：与对照组比较,RES各浓度（50μmol/L和100μmol/L）组均可明显抑制Eca-109细胞侵袭迁移（P〈0.01）,下调N-cadherin、Vimentin、MMP-2、MMP-9 mRNA和蛋白表达（P〈0.05~P〈0.01）,增加E-cadherin mRNA和蛋白表达（P〈0.01）。结论：RES抑制Eca-109细胞侵袭迁移能力,其机制可能通过下调MMP-2、MMP-9的表达,调控EMT蛋白（E-cadherin、N-cadherin和Vimentin）的表达、抑制上皮间质转化过程。Objective： To investigate of the effects of resveratrol（ RES） on the invasion migration of Eca-109 cells and epithelialmesenchymal transition（ EMT） in esophageal squamous cell carcinoma. Methods： The Eca-109 cells were treated with different dilutions（ 50 and 100 μmol / L） of RES,then the wound-healing were performed. The cell migration and invasion was detected by transwell chamber assays. The mRNA and protein expressions of E-cadherin,N-cadherin,Vimentin,MMP-2 and MMP-9 were evaluated using real-time quantitative PCR and Western blot,respectively. Results： Compared with the control group,the other two RES treatment group（ 50 and 100 μmol / L） could significantly inhibit the Eca-109 cell invasion and migration（ P 0. 01）,increase the mRNA and protein expressions of N-cadherin,Vimentin,MMP-2 and MMP-9（ P 0. 05 to P 0. 01）,and increase the mRNA and protein expression of E-cadherin（ P 0. 01）. Conclusions： Resveratrol can inhibit the invasion and migration of Eca-109 cells,which can be caused by down-regulating expressions of the MMP-2 and MMP-9,regulating the expressions of EMT protein（ E-cadherin,N-cadherin and Vimentin）,and inhibiting the epithelial mesenchymal transition process.

关 键 词：食管肿瘤 白藜芦醇 侵袭迁移 上皮-间质转化 

分 类 号：R735.1[医药卫生—肿瘤]