3种培养基中芽囊原虫生长状况观察  

Observation on growth status of Blastocystis hominis in three media

作　　者：俞英昉[1] 吴秀萍[1] 储言红[1] 陈家旭[1] 田利光[1] 

机构地区：[1]中国疾病预防控制中心寄生虫病预防控制所,世界卫生组织疟疾,血吸虫病和丝虫病合作中心,卫生部寄生虫病原与媒介生物学重点实验室,上海200025

出　　处：《中国血吸虫病防治杂志》2016年第1期51-53,65,共4页Chinese Journal of Schistosomiasis Control

基　　金：国家自然科学基金(81473022);上海市卫计委科研课题(140826120622123);国家科技重大专项(2012ZX1004-220)

摘　　要：目的观察芽囊原虫在不同培养基中的生长状况,筛选合适的芽囊原虫培养方法。方法将10份芽囊原虫阳性粪便分别接种至Jone’s液、vitro液、1640 3种培养基中培养,选取生长情况良好、虫密度高的一份粪便传代培养后等量接种至3种不同的培养基中,每24 h计数虫体数量一次,连续观察10 d,并观察芽囊原虫的形态学变化和生长情况。结果10份粪便分别接种至3种培养基中培养,48 h后发现1640和Jone’s液中的虫密度高于vitro液。同一份粪便接种至3种不同培养基中连续观察10 d,结果表明3种培养基中虫体生长呈规律变化,均在接种后3、6、9 d出现生长高峰;Jone’s液中芽囊原虫密度最高。vitro液中观察到的虫体形态最清晰,且富有活力;Jone’s液中能观察到虫体多种繁殖状态。结论Jone’s液适于芽囊原虫的生长繁殖,可作为首选培养基;vitro液可作为观察芽囊原虫生长情况的首选培养基。Objective To observe the growth situation of Blastocystis hominis in vitro and select the optimal method for cultivation of B. hominis in different media. Methods Ten positive stools with B. hominis were inoculated in three different media for cultivating, namely 1640, Jone＇s medium and vitro medium. And the stools with good growth status and high quantities of B. hominis were chosen to inoculate in the three media with equal amount after subcultivation, and the number of B. hominis was counted every 24 h for ten days, and the morphological changes and growth status were also observed. Results The densities of B. hominis in the 1640 and Jone＇ s medium were higher than that in the vitro medium 48 h after the inoculation. The same stool sample was inoculated to the three different media and observed for ten days, and the results indicated that the growth of B. homi- nis presented regular changes in the three media, the growth peaks were on the third, sixth and ninth day post inoculation; and the density of B. hominis was the highest in the Jone＇ s medium. The morphology of B. hominis was the clearest and most dynamic in the vitro medium, while various reproductive forms were observed in the Jone＇ s medium. Conclusion Jone＇ s medium is suitable for the growth of B. hominis and can be the first choice for the cultivation of B. hominis in vitro, and vitro medium is the best medium for observing the growth situation of B. hominis.

关 键 词：芽囊原虫 体外培养 生长状况 形态观察 培养基 Jone’s VITRO 1640 

分 类 号：R382.9[医药卫生—医学寄生虫学]