Association of myostatin Variants with Growth Traits of Zhikong Scallop(Chlamys farreri)  

作　　者：FU Qiang GUO Huihui FENG Liying LI Xue ZHANG Lingling WANG Shi HU Xiaoli BAO Zhenmin 

机构地区：[1]Key Laboratory of Marine Genetics and Breeding (MGB) of Ministry of Education, College of Marine Life Sciences,Ocean University of China, Qingdao 266003, P. R. China

出　　处：《Journal of Ocean University of China》2016年第1期145-151,共7页中国海洋大学学报（英文版）

基　　金：supported by the National High-Tech R&D Program (863 Program,2012AA10A402);Doctoral Fund of Ministry of Education of China (20120132130 002);the National Key Technology R&D Program of China (2011BAD13B06)

摘　　要：Scallop is a popular sea food and an important aquaculture shellfish.Identification of genes and genetic variants relating to scallop growth could benefit high-yielding scallop breeding.Myostatin(MSTN) is a conservative regulator of muscle growth,and has become one of the most important target genes for genetic improvement of the production of farmed animals.In this study,four single nucleotide polymorphisms(SNPs) were identified in the 5' flanking region of MSTN gene(Cf MSTN) in Zhikong scallop(Chlamys farreri).The association of these SNPs with scallop growth traits,including shell length,shell height,body weight and striated muscle weight was analyzed.The SNP g-1162G>T was found to associate with shell length,shell height,and striated muscle weight.The TT type scallops showed significantly higher trait values than those of GT type,and the GG type individuals exhibited median values.On the contrary,significantly more Cf MSTN transcripts were detected in the striated muscle of GT type scallops than in those of TT and GG type ones.Our results suggested that Cf MSTN might regulate the scallop muscle growth negatively,and SNP g-1162G>T can be used as a candidate marker for the selective breeding of high-yielding scallop.Scallop is a popular sea food and an important aquaculture shellfish. Identification of genes and genetic variants relating to scallop growth could benefit high-yielding scallop breeding. Myostatin （MSTN） is a conservative regulator of muscle growth, and has become one of the most important target genes for genetic improvement of the production of farmed animals. In this study, four single nucleotide polymorphisms （SNPs） were identified in the 5＇ flanking region of MSTN gene （CfMSTN） in Zhikong scallop （Chlamys farreri）. The association of these SNPs with scallop growth traits, including shell length, shell height, body weight and striated muscle weight was analyzed. The SNP g-1162G〉T was found to associate with shell length, shell height, and striated muscle weight. The TT type scallops showed significantly higher trait values than those of GT type, and the GG type individuals exhibited median values. On the contrary, significantly more CfMSTN transcripts were detected in the striated muscle of GT type scallops than in those of TT and GG type ones. Our results suggested that CfMSTN might regulate the scallop muscle growth negatively, and SNP g-1162G〉T can be used as a candidate marker for the selective breeding of high-yielding scallop.

关 键 词：Zhikong scallop Chlamys farreri MYOSTATIN single nucleotide polymorphism growth trait EXPRESSION association analysis 

分 类 号：S917.4[农业科学—水产科学]