2-脱氧葡萄糖对体外培养人γδT细胞CCR5表达及杀伤功能影响  

Effect of 2-deoxy-D-glucoseon on expression of CCR5 and killing function of human γδT cells in vitro

作　　者：郑璐[1] 陈永强[1] 刘军权[1] 吕小婷[1] 张娟[1] 孙蕾清[1] 徐晶[1] 周忠海[1] 陈复兴[1] 

机构地区：[1]解放军第97医院中心实验室,徐州221004

出　　处：《中国免疫学杂志》2016年第1期29-32,共4页Chinese Journal of Immunology

基　　金：南京军区医学科技创新研究基金(14MS032)资助

摘　　要：目的：观察糖基化抑制剂2-脱氧葡萄糖（2-deoxy-D-glucose,2-DG）对γδT细胞增殖、趋化因子受体CCR5和杀伤功能的影响。方法：从健康人外周血中分离单个核细胞,加入到γδT细胞完全培养基培养获得γδT细胞。用不同浓度的2-DG诱导培养γδT细胞48 h后,用CCK-8法测定不同浓度2-DG组的γδT细胞增殖和杀伤能力;用流式细胞术检测γδT细胞CCR5的表达和杀伤功能指标的影响。结果：培养10 d后的γδT细胞纯度达到（88.18±3.41）%。2-DG浓度在0~1.0 mmol/L时对γδT细胞的增殖影响不明显,而当浓度大于1.0 mmol/L后能显著抑制γδT细胞的增殖。2-DG浓度在0~2.0 mmol/L范围内能促进CCR5的表达,且在0.5和1.0 mmol/L时能促进杀伤指标CD107a和穿孔素的表达及对结肠癌HCT116细胞的杀伤能力。结论：2-DG能促进γδT细胞表面趋化因子受体CCR5的表达,且在一定浓度范围内能提高γδT细胞的体外杀伤功能。Objective：To investigate the effect of 2-deoxy-D-glucose （2-DG） on hunman γδT cells on the expression of CCR5 and killing function in vitro. Methods ： Using γδT medium to cultivate peripheral blood mononuclear cell （PBMCs） in vitro. After co- cultured with various concentrations of 2-DG for 48 h, the expression of CCR5 and killing activities of γδT ceils for each group were detected by flow cytometry and CCK-8 methods. Results： 2-DG could not promote the growth of 3,8T cells with the increase in concentration from 0 μmol/L to 1.0 μmol/L and decreased thereafter. The certain concentration （0-2.0 μmol/L） of 2-DG could upregulate the expression of CCR5 in dose dependent manner. Besides, at 0. 5 μmol/L and 1.0 μmol/L of 2-DG could increase the ex- pression of CDIOTa and perforin and have no effect on the granzyme B. Conclusion： Human γδT cells isolated from peripheral blood treated with 2-DG could promote the expression of CCR5 and increase the killing activities at certain concentration in vitro.

关 键 词：2-DG ΓΔT细胞 CCR5 杀伤功能 

分 类 号：R392.12[医药卫生—免疫学]