正常及肺气虚大鼠肺组织蛋白组学差异分析  

Normal and lung qi deficiency rats proteomics of lung tissue variance analysis

作　　者：王平[1] 刘芳芳[1] 李俊莲[1] 马彦平[1] 张晓薇[1] 李艳彦[1] 张维骏[1] 陶功定[1] 

机构地区：[1]山西中医学院,太原030619

出　　处：《中国免疫学杂志》2016年第1期46-50,共5页Chinese Journal of Immunology

基　　金：国家自然科学基金资助项目(81173155)

摘　　要：目的:探讨正常及肺气虚大鼠肺组织蛋白组学差异表达及质谱分析。方法:选取20只SD大鼠随机分为正常对照组与肺气虚组每组10只。肺气虚组模型复制采用气管注射脂多糖与烟熏的复合方法。造模28 d后,两组大鼠于麻醉前12 h禁食、禁水,称量体重。麻醉后开胸取出左肺组织10 mg/只,每组取3个样本进行样品蛋白质制备、双向电泳实验、图像分析、质谱检测。结果:对两组差异蛋白图谱进行蛋白质组学分析,鉴定出14个蛋白在两组之间差异表达,肺气虚组与正常对照组比较,其中5个表达上调、9个表达下调。结论:通过蛋白质组学分析,鉴定出的蛋白与肺气虚证有相关性。Objective：Normal and lung qi deficiency rats of proteomics of lung tissue of rats differentially expressed and mass spectrometry. Methods： A total of 20 SD rats were randomly divided into control group, lung qi deficiency group, with 10 rats in each roup. Lung qi deficieney group model replication using composite method by intratracheal injection of lipopolysaccaride and smoked. All rats in each group before anesthesia 12 hours of fasting, water deprivation, weight after 28 days modeling,Thoracotomy to remove left lung tissue after anesthesia was 10 mg from each rat, 3 rats from each group, using two-dimensional electrophorsis analysis, two- dimensional gel electrophoresis image, peptide mass fingerprinting and electrospray tandem by mass spectrometry to protein identification. Results： 2 differntial protin profiles for proteomics analysis,identified 14 protein in rat lung tissue, protein 5 expression, regulation 9 expression. Conclusion： By proteomic analysis,identify the protein have correlation with lung deficiency syndrome

关 键 词：肺气虚 肺组织 蛋白组学 差异分析 

分 类 号：R22[医药卫生—中医基础理论]