白藜芦醇通过抑制内质网应激减轻大鼠脑缺血/再灌注损伤  

Resveratrol ameliorates cerebral ischemia/reperfusion injury by inhibiting endoplasmic reticulum stress in rats

作　　者：高轩[1] 陈荔枝 刘振红[2] 吕艳华[2] 夏立伟[1] 李永秋[1] 

机构地区：[1]唐山市工人医院神经内科,唐山063000 [2]唐山市工人医院社区医疗部,唐山063000

出　　处：《中国免疫学杂志》2016年第1期92-96,共5页Chinese Journal of Immunology

摘　　要：目的:探讨白藜芦醇(Resveratrol,Res)对大鼠脑缺血/再灌注诱导的内质网应激反应的调控作用。方法:将72只健康雄性SD大鼠,采用随机数字表法分为3组(n=24):假手术组(S组)、脑缺血/再灌注模型组(I/R组)、Res组(R组)。采用阻断左侧大脑中动脉并阻断双侧颈总动脉30 min恢复灌注的方法制备大鼠脑缺血/再灌注模型。R组于缺血前7d给予腹腔注射Res(50 mg/kg),1次/日。对大鼠进行神经功能缺损评分后留取脑组织,采用TTC法检测脑梗死体积,干湿重法测定脑组织含水量,免疫组织化学及Western blot法检测大脑皮层内质网应激相关蛋白GRP78、p-PERK、CHOP的表达变化。结果:与S组比较,I/R组大鼠神经功能评分及脑含水量升高(P<0.05),脑梗死体积增大(P<0.05),皮层GRP78、p-PERK和CHOP蛋白表达均显著上调(P<0.05)。与I/R组比较,P组大鼠神经功能评分及脑含水量降低(P<0.05),脑梗死体积减小(P<0.05),GRP78表达上调(P<0.05),p-PERK和CHOP蛋白表达下调(P<0.05)。结论:白藜芦醇通过抑制内质网应激反应,对大鼠脑缺血/再灌注损伤发挥保护作用。Objective ： To investigate the effect of resveratrol （Res） on endoplasmic reticulum stress induced by cerebral ischemia/reperfusion（I/R） injury in rats. Methods： The seventy-two male SD rats were divided randomly into three groups （n = 20） ： sham operation group （group S ）, I/R group and Res-treatment group （group R ）. Focal cerebral I/R model was induced by electrocoagulation of left middle cerebral artery and occlusion of bilateral common carotid arteries followed by reperfusion after 30 min. The rats in Res group were treated with Res（50 mg/kg）i, p. 7 d before the operation,once a day for 7 d. Neurological deficits were assessed at 24 h post-injury,followed by collecting the brain tissues. Cerebral infarct size was detected by TIC staining,and the water content of brain tissue were measured by wet-dry weight method. The expression of GRP78, p-PERK and CHOP proteins were deter- mined by immunohistochemistry and Western blot analysis. Results： Compared with sham group, the neurological deficit score and the brain water content were significantly increased（ P〈0.05 ）, cerebral infarct size was enlarged （P〈0. 05 ）, and the expression of GRP78, p-PERK and CHOP were up-regulated in I/R group（P〈0. 05 ）. At the corresponding time, compared with I/R group, the neurological deficit score and the brain water content were markedly decreased（P〈0.05 ）, cerebral infarct size was smaller（ P〈0.05 ）, the level of GRF78 was notablely increased（ P〈0.05 ）, while the expression of p-PERK and CHOP were down-regulated in Res group （P〈0. 05 ）. Conclusion： Resveratrol plays a protection role in ischemia-reperfusion injury, through inhibiting the endoplasmic reticulum stress in rats.

关 键 词：白藜芦醇 脑缺血 再灌注损伤 内质网 应激 

分 类 号：R743.35[医药卫生—神经病学与精神病学]