他莫昔芬对人胃癌细胞增殖和丝氨酸蛋白酶抑制剂9表达的影响  

Effects of Tamoxifen on proliferation and expression of serine proteinase inhibitor 9 in human gastric cancer cells

作　　者：陈海霞[1] 周业江[2] 王璐璐[1] 李华[1] 熊玉霞[1] 

机构地区：[1]泸州医学院药学院药理教研室,四川泸州646000 [2]泸州医学院附属医院普外科,四川泸州646000

出　　处：《重庆医学》2016年第7期873-875,879,共4页Chongqing medicine

基　　金：国家自然科学基金资助项目(30672058);泸州医学院科研基金资助项目(2009246)

摘　　要：目的 研究他莫昔芬（TAM）对胃癌细胞雌激素受体α、β（ERα和ERβ）的表达情况及对胃癌细胞增殖和丝氨酸蛋白酶抑制剂9（PI9）表达的影响。方法 对前期筛选出的ERα表达阳性胃癌细胞株MNK45、SGC7901（PI9表达阳性）和ERβ表达阳性胃癌细胞株BGC823（PI9表达阴性）,采用免疫荧光化学法检测TAM对ERα和ERβ表达的影响,CCK8法检测TAM干预后胃癌细胞株的增殖情况,逆转录PCR观察TAM干预后PI9表达的变化。结果 ERα表达于MNK45和SGC7901细胞核上,ERβ表达于BGC823细胞核上,经TAM干预后ERα和ERβ表达均无明显改变;TAM在0.1~100.0μmol/L时可明显抑制SGC7901、MNK45的细胞增殖,与阴性对照组比较,差异有统计学意义（P〈0.05）,对BGC823和MNK28的细胞增殖无明显剂量依赖关系;TAM干预后SGC7901的PI9mRNA的相对灰度值为（0.402±0.020）,MNK45的PI9mRNA的相对灰度值为（0.359±0.048）,较阴性对照组灰度值均明显降低,差异有统计学意义（P〈0.05）。结论 TAM对ERα和PI9阳性表达细胞的增殖抑制作用强于PI9阴性表达细胞,并呈较好的剂量依赖性;TAM对胃癌的生长抑制作用可能与改善PI9介导的免疫耐受有关。Objective To observe the expression of estrogen receptors（ERαand ERβ）on gastric cancer cells and evaluate the effect of Tamoxifen（TAM）on the cell proliferation and expression of serine proteinase inhibitor 9（PI9）of gastric cancer cells.Methods PI9 positive expression（MNK45,SGC7901）and negative expression（BGC823）of gastric cancer cell lines were from preliminary screened,the expression of ERαand ERβdetected by immunofluorescence chemical method,the cell proliferation and expression of PI9 were tested by CCK8 assay and reverse transcription-PCR after intervention of TAM.Results ERαprotein expression was noted in MNK45 and SGC7901,ERβwas noted in BGC823,but the expression of ERαand ERβwere not appear to be obvious after the intervention of TAM.Tamoxifen could obviously inhibited cell proliferation of MNK45 and SGC7901at concentration of 0.1-100.0μmol/L,the differences were statistically significant compared with negative control group（P〈0.05）,but showed no dose-dependent to the proliferation of BGC823 and MNK28.After treating with TAM,the expression of PI9 mRNA of SGC7901（0.402±0.020）and MNK45（0.359±0.048）were obviously lower than that in the negatwe control group（P〈 0.05）.Conclusion Tamoxifen could significantly inhibit the proliferation of PI9 positive expression than PI9 negative expression of gastric cancer cell lines,and showed obviously dose-dependent,its role in inhibiting proliferation might closely related to immune tolerance improved by PI9.

关 键 词：胃肿瘤 受体 雌激素 他莫昔芬 丝氨酸蛋白酶抑制剂9 

分 类 号：R735.2[医药卫生—肿瘤]